About: Assessment of diversity of Czech barley cultivars as revealed by PCR markers, SSR, AFLP and DNA assays

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We used a set of DNA markers to characterise malting barley cultivars used in the Czech Republic in the last century. SSR and AFLP markers revealed a shift in the genetic basis of currently used cultivars in comparison with obsolete cultivars. Principal component analysis showed that the gene-pool in use has narrower genetic basis. The results are supported by the analysis of the data released from repetitive field trials. TDI-FRET based analysis of beta-amylase alleles has led to discovery of highly thermostable allele Sd2H in modern local adopted malting barley cultivar 'Malvaz', which can be used further in breeding programmes. Other thermostable alleles Sd3 were found in old genetic resources. The results show that molecular analysis can reveal increased value of genetic resources We used a set of DNA markers to characterise malting barley cultivars used in the Czech Republic in the last century. SSR and AFLP markers revealed a shift in the genetic basis of currently used cultivars in comparison with obsolete cultivars. Principal component analysis showed that the gene-pool in use has narrower genetic basis. The results are supported by the analysis of the data released from repetitive field trials. TDI-FRET based analysis of beta-amylase alleles has led to discovery of highly thermostable allele Sd2H in modern local adopted malting barley cultivar 'Malvaz', which can be used further in breeding programmes. Other thermostable alleles Sd3 were found in old genetic resources. The results show that molecular analysis can reveal increased value of genetic resources (en) Využili jsme molekulární markéry k charakterizci diverzity odrůd sladovnického ječmene používaných v minulém století na území ČR a SR. PCA založená na AFLP a SSR markérech prokázala posun v genetickém základutéto plodiny. Rovněž jsme detekovali několik nositelů termostabilní alely beta-amylázy. Výsledky ukazují, že molekulární markéry jsou vhodným násetrojem pro hodnocení diverzity. (cs)
Title	Assessment of diversity of Czech barley cultivars as revealed by PCR markers, SSR, AFLP and DNA assays Odhad diverzity českých kultivarů ječmene zjištěný PCR markery, SSR, AFLP a DNA zkouškami (cs) Assessment of diversity of Czech barley cultivars as revealed by PCR markers, SSR, AFLP and DNA assays (en)
skos:prefLabel	Assessment of diversity of Czech barley cultivars as revealed by PCR markers, SSR, AFLP and DNA assays Odhad diverzity českých kultivarů ječmene zjištěný PCR markery, SSR, AFLP a DNA zkouškami (cs) Assessment of diversity of Czech barley cultivars as revealed by PCR markers, SSR, AFLP and DNA assays (en)
skos:notation	RIV/00027006:_____/04:176!RIV08-MZE-00027006
http://linked.open.../vavai/riv/strany	39;48
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(QC1336), P(QC1345)
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Kučera, Ladislav Ovesná, Jaroslava Machová Poláková, Kateřina
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav rostlinné výroby, v.v.i.
http://linked.open...dnocenehoVysledku	555450
http://linked.open...ai/riv/idVysledku	RIV/00027006:_____/04:176
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	barley; genetic resources; malting quality; DNA markers; SSR; AFLP (en)
http://linked.open.../riv/klicoveSlovo	SSR genetic resources DNA markers barley malting quality AFLP
http://linked.open...ontrolniKodProRIV	[E8D5B55C520E]
http://linked.open...v/mistoKonaniAkce	Brno
http://linked.open...i/riv/mistoVydani	Kromeriz
http://linked.open...i/riv/nazevZdroje	9th International Barley Genetics Symposium
http://linked.open...in/vavai/riv/obor	GE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Development and employment of new approaches for monitoring of genetic diversity and large-scale screening of genetic resources Enhancement of the value of wheat and barley collections and improvement of their management
http://linked.open...UplatneniVysledku	2004
http://linked.open...iv/tvurceVysledku	Kučera, Ladislav Ovesná, Jaroslava Vaculová, K. Milotová, J. Machová-Poláková, Kateřina
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2004-06-20 (xsd:date)
number of pages	10 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Agriculture Research Institute Kromeriz Ltd.
https://schema.org/isbn	80-902545-9-4

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