About: SNP Typing in Cereals: Comparison of SSCP and SN aPShot Markers Using the Barley Mlo Locus as a Model

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About: SNP Typing in Cereals: Comparison of SSCP and SN aPShot Markers Using the Barley Mlo Locus as a Model Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Porovnávali jsme využitelnost SSCP a SNPaShot markérů na Mlo lokusu ječmene jako modelu pro možnost detekce bodových mutací u obilovin. Zatímcovývoj SSCP markérů vyžaduje několik kroků optimalizace SNPaShot markéry založené na jednokrokovém prodloužení řetězce DNA o jeden fluorescenčně značený nukleotid vyžaduje pouze předběžnou znalost daé sekvence. Jednobodové mutace mohou být jednoduše detekovány pomocí kapilární elektroforézy (cs) We compared the applicability of SSCP and SNaPShot markers in barley Mlo loci as a model of SNP detection in cereals. Whereas the development of SSCP markers required optimisation steps, the ddNTP primer extension (SNaPShot) procedure based onknowledge of target sequence provided expected results without any previous optimisations. We have shown, that SNPs can be easily scored using the ABI PRISM 310 Genetic Ana- lyser. We compared the applicability of SSCP and SNaPShot markers in barley Mlo loci as a model of SNP detection in cereals. Whereas the development of SSCP markers required optimisation steps, the ddNTP primer extension (SNaPShot) procedure based onknowledge of target sequence provided expected results without any previous optimisations. We have shown, that SNPs can be easily scored using the ABI PRISM 310 Genetic Ana- lyser. (en)
Title	SNP Typing in Cereals: Comparison of SSCP and SN aPShot Markers Using the Barley Mlo Locus as a Model SNP Typing in Cereals: Comparison of SSCP and SN aPShot Markers Using the Barley Mlo Locus as a Model (en) Genotypizace SNP u obilovin: porovnání SSCP a SNaPShot markerů na modelu Mlo lokusu ječmene (cs)
skos:prefLabel	SNP Typing in Cereals: Comparison of SSCP and SN aPShot Markers Using the Barley Mlo Locus as a Model SNP Typing in Cereals: Comparison of SSCP and SN aPShot Markers Using the Barley Mlo Locus as a Model (en) Genotypizace SNP u obilovin: porovnání SSCP a SNaPShot markerů na modelu Mlo lokusu ječmene (cs)
skos:notation	RIV/00027006:_____/03:96!RIV08-MZE-00027006
http://linked.open.../vavai/riv/strany	109;112
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(QD1335)
http://linked.open...iv/cisloPeriodika	39
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Rulcová-Karavdić, Jana Kučera, Ladislav Ovesná, Jaroslava Machová Poláková, Kateřina
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav rostlinné výroby, v.v.i.
http://linked.open...dnocenehoVysledku	627494
http://linked.open...ai/riv/idVysledku	RIV/00027006:_____/03:96
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	SSCP; SNaPShot; Mlo; SNP; DNA marker; barley; primer extension (en)
http://linked.open.../riv/klicoveSlovo	DNA marker SNP SSCP barley Mlo primer extension SNaPShot
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[ECB135567D0D]
http://linked.open...i/riv/nazevZdroje	Czech Journal of Genetics and Plant Breeding
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of new DNA marker types and evaluation of their potetial for barley genome investigation, marker assisted selection and genotype identification
http://linked.open...UplatneniVysledku	2003
http://linked.open...v/svazekPeriodika	2003
http://linked.open...iv/tvurceVysledku	Kučera, Ladislav Ovesná, Jaroslava Machová-Poláková, Kateřina Rulcová, Jana
issn	1212-1975
number of pages	4 (xsd:int)

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