About: A rapid and effective RNA release procedure for virus detection in woody plants by reverse trancription-polymerase chain reaction

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About: A rapid and effective RNA release procedure for virus detection in woody plants by reverse trancription-polymerase chain reaction Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Rychlá a účinná metoda uvolnění RNA (RNA-RP) pro detekci ASPV a ASGV u jádrovin pomocí RT-PCR byla vyvinuta. RNA-RP byla porovnána s klasickou fenol-chloroformovou extrakcí RNA. Citlivost RT-PCR u obou metod extrakce byla podobná. (cs) A rapid and effective RNA release procedure (RNA-RP) for detection of the Apple stem pitting virus (ASPV) and the Apple stem grooving virus (ASGV) in woody plants by reverse transcription-polymerase chain reaction (RT-PCR) was developed. RNA-RP released RNA into crude homogenate. RNA-RP was compared with classical phenol/chlorophorm extraction of RNA. The RT-PCR detection of ASPV and ASGV was shown to be similar by both the RNA preparation procedures. RNA-RP in contrast to the classical phenol/chloroform procedure represents a reliable and easy-to band protocol convenient for routine virus detection. Leaf tissues, especially dormant bud leaves and leaves during blossom, were shown to be the most optimal material for detection of these viruses, irrespective of the used RNA preparation procedure. A rapid and effective RNA release procedure (RNA-RP) for detection of the Apple stem pitting virus (ASPV) and the Apple stem grooving virus (ASGV) in woody plants by reverse transcription-polymerase chain reaction (RT-PCR) was developed. RNA-RP released RNA into crude homogenate. RNA-RP was compared with classical phenol/chlorophorm extraction of RNA. The RT-PCR detection of ASPV and ASGV was shown to be similar by both the RNA preparation procedures. RNA-RP in contrast to the classical phenol/chloroform procedure represents a reliable and easy-to band protocol convenient for routine virus detection. Leaf tissues, especially dormant bud leaves and leaves during blossom, were shown to be the most optimal material for detection of these viruses, irrespective of the used RNA preparation procedure. (en)
Title	Rychlá a efektivní metoda RNA release pro detekci viru dřevin pomocí RT-PCR (cs) A rapid and effective RNA release procedure for virus detection in woody plants by reverse trancription-polymerase chain reaction A rapid and effective RNA release procedure for virus detection in woody plants by reverse trancription-polymerase chain reaction (en)
skos:prefLabel	Rychlá a efektivní metoda RNA release pro detekci viru dřevin pomocí RT-PCR (cs) A rapid and effective RNA release procedure for virus detection in woody plants by reverse trancription-polymerase chain reaction A rapid and effective RNA release procedure for virus detection in woody plants by reverse trancription-polymerase chain reaction (en)
skos:notation	RIV/00027006:_____/03:31!RIV08-MZE-00027006
http://linked.open.../vavai/riv/strany	147;151
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA522/00/D025), Z(MZE-M01-01-03)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Kumar, Jiban
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav rostlinné výroby, v.v.i.
http://linked.open...dnocenehoVysledku	597097
http://linked.open...ai/riv/idVysledku	RIV/00027006:_____/03:31
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	ASPV; ASGV; RT-PCR (en)
http://linked.open.../riv/klicoveSlovo	RT-PCR ASGV ASPV
http://linked.open...odStatuVydavatele	SK - Slovenská republika
http://linked.open...ontrolniKodProRIV	[CC2513982960]
http://linked.open...i/riv/nazevZdroje	Acta Virologica
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	1 (xsd:int)
http://linked.open...vavai/riv/projekt	Molecular and biological diagnosis of apple stem pitting virus (ASPV) in the Czech Republic
http://linked.open...UplatneniVysledku	2003
http://linked.open...v/svazekPeriodika	47
http://linked.open...iv/tvurceVysledku	Kumar, Jiban
http://linked.open...n/vavai/riv/zamer	Výzkum ekologie, epidemiologie, šíření a metody diagnostiky škodlivých organismů, inovace systémů ochrany rostlin a skladovaných produktů
issn	0001-723X
number of pages	5 (xsd:int)

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