| Attributes | Values |
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| rdf:type
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| Description
| - Čtyři bílkovinné frakce (1 – albuminy a globuliny), 2 – gliadiny, 3 – gluteniny (extrahované pomocí NaOH), 4 – gluteniny (extrahované pomocí SDS) byly děleny v podmínkách SDS na polyakrylamidovém gelu a použity jako biochemické markery pro hodnocení stupně polymorfismu u tří odrůd pšenice špaldy – Hercule, Altgold a Rouquin a tří novošlechtění – H92.27, H92.28 a M92.20 (vytvořených křížením pšenice špaldy s pšenicí setou) a referenční odrůdy pšenice seté Brea. Výsledné elektroforeogramy byly digitalizovány a pomocí Nei a Li koeficientu podobnosti byl vyhodnocen vztah mezi sledovanými genotypy. Celkové hodnocení všech čtyř markerovacích systémů vykazovalo diference mezi pšenicí setou Brea, odrůdami špaldy i hybridními liniemi. Rovněž byly zjištěny významné diference mezi původními starými odrůdami pšenice špaldy (Hercule, Altgold a Rouquin) a hybridními liniemi pšenice špaldy. V podmínkách Osbornovy extrakce byla potvrzena skutečnost vzájemného přechodu proteinových frakcí (gliadinů a gluteninů). Z (cs)
- Four protein fractions: 1 – albumins and globulins, 2 – gliadins, 3 – glutenins (extracted in NaOH), 4 – glutenins (extracted in SDS) separated by SDS-PAGE were used as biochemical markers for evaluation of polymorphism level in three spelt wheat cultivars – Hercule, Altgold a Rouquin, three new–breeders’ spelt lines – H92.27, H92.28 a M92.20 (originated from hybridisation between spelt and common wheat) and reference common wheat cultivar Brea. Electrophoretic phenotypes and zymograms were evaluated by means of digital image analysis and Nei and Li coefficient of similarity was used to evaluate the relation of analysed genotypes. Entire evaluation of all four-marker systems showed differences between common wheat cultivar Brea and spelt cultivars and spelt breeders’ lines. Also significant differences between old spelt cultivars (Hercule, Altgold and Rouquin) and new spelt breeders’ lines were found. The reality of the mutual passing of protein fractions (gliadins and glutenins), based on Osborne e
- Four protein fractions: 1 – albumins and globulins, 2 – gliadins, 3 – glutenins (extracted in NaOH), 4 – glutenins (extracted in SDS) separated by SDS-PAGE were used as biochemical markers for evaluation of polymorphism level in three spelt wheat cultivars – Hercule, Altgold a Rouquin, three new–breeders’ spelt lines – H92.27, H92.28 a M92.20 (originated from hybridisation between spelt and common wheat) and reference common wheat cultivar Brea. Electrophoretic phenotypes and zymograms were evaluated by means of digital image analysis and Nei and Li coefficient of similarity was used to evaluate the relation of analysed genotypes. Entire evaluation of all four-marker systems showed differences between common wheat cultivar Brea and spelt cultivars and spelt breeders’ lines. Also significant differences between old spelt cultivars (Hercule, Altgold and Rouquin) and new spelt breeders’ lines were found. The reality of the mutual passing of protein fractions (gliadins and glutenins), based on Osborne e (en)
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| Title
| - Hodnocení bílkovinných frakcí jako biochemických markerů pro identifikaci odrůd pšenice špaldy (Triticum spelta L.) (cs)
- Evaluation of protein fractions as biochemical markers for identification of spelt wheat cultivars (Triticum spelta L.)
- Evaluation of protein fractions as biochemical markers for identification of spelt wheat cultivars (Triticum spelta L.) (en)
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| skos:prefLabel
| - Hodnocení bílkovinných frakcí jako biochemických markerů pro identifikaci odrůd pšenice špaldy (Triticum spelta L.) (cs)
- Evaluation of protein fractions as biochemical markers for identification of spelt wheat cultivars (Triticum spelta L.)
- Evaluation of protein fractions as biochemical markers for identification of spelt wheat cultivars (Triticum spelta L.) (en)
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| skos:notation
| - RIV/00027006:_____/03:1588!RIV08-GA0-00027006
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00027006:_____/03:1588
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - spelt wheat, biochemical markers, seed proteins, electrophoresis, protein fractions (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Plant, Soil and Environment
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Dvořáček, Václav
- Čurn, Vladislav
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| issn
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| number of pages
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