About: Selection of RNA isolation method for molecular detection of grapevine viruses     Goto   Sponge   NotDistinct   Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

AttributesValues
rdf:type
Description
  • Byly vybrány révy infikované virem spojeným se svinutkou révy vinné 1 (GLRaV-1) a virem spojeným se svinutkou révy vinné 3 (GLRaV-3). Z těchto rostlin byly odebrány vzorky dormantního réví, z jehož lýkového pletiva byla izolována celková RNA použitím tří různých metod: metody používající močovinový pufr a následnou extrakci směsí fenol-chloroform, metody používající reagencii Concert firmy Invitrogen a metody používající kit RNeasy Plant Mini Kit od firmy Qiagen. Nejvyšší výtěžek RNA byl dosažen s použitím reagencie Concert. Při použití takto izolované RNA v RT-PCR byly snadno detekovány oba viry, GLRaV-1 i GLRaV-3, zatímco u RNA izolovaných jinými metodami nebylo možno tyto viry detekovat. (cs)
  • Grapevines infected with Grapevine leafroll-associated virus-1 (GLRaV-1) and Grapevine leafroll-associated virus-3 (GLRaV-3) were selected. Total RNA was isolated from grapevine phloem tissue scrapped from dormant canes by three different methods: extraction with urea buffer followed with phenol-chloroform extraction, method using Concert reagent (Invitrogen) followed with chloroform-isopropylalcohol extraction, and procedure using RNeasy Plant Mini Kit (Qiagen). The highest yield of RNA was obtained using ConcertTM reagent. If this RNA was used in RT-PCR, GLRaV-1 and GLRaV-3 were easily detected. From RNA isolated by other two methods these viruses were not detected.
  • Grapevines infected with Grapevine leafroll-associated virus-1 (GLRaV-1) and Grapevine leafroll-associated virus-3 (GLRaV-3) were selected. Total RNA was isolated from grapevine phloem tissue scrapped from dormant canes by three different methods: extraction with urea buffer followed with phenol-chloroform extraction, method using Concert reagent (Invitrogen) followed with chloroform-isopropylalcohol extraction, and procedure using RNeasy Plant Mini Kit (Qiagen). The highest yield of RNA was obtained using ConcertTM reagent. If this RNA was used in RT-PCR, GLRaV-1 and GLRaV-3 were easily detected. From RNA isolated by other two methods these viruses were not detected. (en)
Title
  • Metoda izolace RNA pro detekci virů z pletiv révy vinné (cs)
  • Selection of RNA isolation method for molecular detection of grapevine viruses
  • Selection of RNA isolation method for molecular detection of grapevine viruses (en)
skos:prefLabel
  • Metoda izolace RNA pro detekci virů z pletiv révy vinné (cs)
  • Selection of RNA isolation method for molecular detection of grapevine viruses
  • Selection of RNA isolation method for molecular detection of grapevine viruses (en)
skos:notation
  • RIV/00027006:_____/02:1280!RIV07-GA0-00027006
http://linked.open.../vavai/riv/strany
  • 267;270
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GP522/01/D131)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 663192
http://linked.open...ai/riv/idVysledku
  • RIV/00027006:_____/02:1280
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Grapevine leafroll-associated virus 1, Grapevine leafroll-associated virus 3, grapevine, ELISA, RT-PCR (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [FD6556A90389]
http://linked.open...v/mistoKonaniAkce
  • Praha
http://linked.open...i/riv/mistoVydani
  • Praha
http://linked.open...vEdiceCisloSvazku
  • Neuvedeno, 0
http://linked.open...i/riv/nazevZdroje
  • Plant Protection Science, Proceedings 6th Conference of EFPP 2002
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Komínek, Petr
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
issn
  • 1212-2580
number of pages
http://purl.org/ne...btex#hasPublisher
  • Institute of Agricultural and Food Information
Faceted Search & Find service v1.16.118 as of Jun 21 2024


Alternative Linked Data Documents: ODE     Content Formats:   [cxml] [csv]     RDF   [text] [turtle] [ld+json] [rdf+json] [rdf+xml]     ODATA   [atom+xml] [odata+json]     Microdata   [microdata+json] [html]    About   
This material is Open Knowledge   W3C Semantic Web Technology [RDF Data] Valid XHTML + RDFa
OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 58 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software