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Description
| - Monoclonal antibody MN-CS1 with high level of sensitivity and specificity for detection and determination of Clavibacter michiganensis subsp. sepedonicus was prepared. Strain Clavibacter michiganensis subsp. sepedonicusNCPPB 3467 (as whole cell antigen and extracellulare polysaccharides) was used for immunisation of four mice Balb/c. After cloning and verifiing, two stable hybridoma were gained. One monoclonal antibody Mn-Cs1 was used in all tests. It reacted intensively with extracellular polysaccharides from homologous antigen (more than 0,5 mg/ml), weakly with proteins from cell walls and with whole antigen in DAS-ELISA. Monoclonal antibody Mn-Cs1 showed high level of specificity. It reacted neither with bacterial strains of closely related suspecies of Clavibacter michiganensis nor with the saprophytic bacteria Pseudomonas fluorescens and Pantoea agglomerans.
- Monoclonal antibody MN-CS1 with high level of sensitivity and specificity for detection and determination of Clavibacter michiganensis subsp. sepedonicus was prepared. Strain Clavibacter michiganensis subsp. sepedonicusNCPPB 3467 (as whole cell antigen and extracellulare polysaccharides) was used for immunisation of four mice Balb/c. After cloning and verifiing, two stable hybridoma were gained. One monoclonal antibody Mn-Cs1 was used in all tests. It reacted intensively with extracellular polysaccharides from homologous antigen (more than 0,5 mg/ml), weakly with proteins from cell walls and with whole antigen in DAS-ELISA. Monoclonal antibody Mn-Cs1 showed high level of specificity. It reacted neither with bacterial strains of closely related suspecies of Clavibacter michiganensis nor with the saprophytic bacteria Pseudomonas fluorescens and Pantoea agglomerans. (cs)
- Monoklonální protilátka MN-CS1 vykázala vysokou hladinu citlivosti a specifičnosti.Monoklonální protilátka MN-CS1 byla připravena proti Clavibacter michiganensis subsp. sepedonicus NCPPB 3467 (celobuněčnému antigenu extracelulárním polysacharidům). Bylyzískány dva stabilní hybridomy. Monoklonální protilátka MN-CS1 byla použita ve všech sérologických testech. Protilátka reagovala intensivně s extracelulárními polysacharidy homologního antigenu, naopak reagovala slabě s proteiny buněčné stěny a celobuněčným antigenem v DAS-ELISA. Monoklonální protilátka MN-CS1 nereagovala s bakteriálními kmeny z příbuzných poddruhů Clavibacter michiganensis ani s bakteriemi Pseudomonas fluorescens and Pantoea agglomerans. (en)
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Title
| - Sensitivity and specificity of monoclonal antibody Mn-Cs1 for detection and determination of Clavibacter michiganensis subsp. sepedonicus, the causal agent of bacterial ring rot of potato (en)
- Citlivost a specifita monoklonální protilátky Mn-Cs1 pro detekci a determinaci Clavibacter michiganensis subsp. sepedonicus, původce bakteriální hniloby brambor
- Citlivost a specifita monoklonální protilátky Mn-Cs1 pro detekci a determinaci Clavibacter michiganensis subsp. sepedonicus, původce bakteriální hniloby brambor (cs)
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skos:prefLabel
| - Sensitivity and specificity of monoclonal antibody Mn-Cs1 for detection and determination of Clavibacter michiganensis subsp. sepedonicus, the causal agent of bacterial ring rot of potato (en)
- Citlivost a specifita monoklonální protilátky Mn-Cs1 pro detekci a determinaci Clavibacter michiganensis subsp. sepedonicus, původce bakteriální hniloby brambor
- Citlivost a specifita monoklonální protilátky Mn-Cs1 pro detekci a determinaci Clavibacter michiganensis subsp. sepedonicus, původce bakteriální hniloby brambor (cs)
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skos:notation
| - RIV/00027006:_____/02:#####488!RIV06-MZE-00027006
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00027006:_____/02:#####488
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - bakteriální hniloba brambor, Clavibacter michiganensis subsp. sepedonicus, DAS-ELISA (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Kokošková, Blanka
- Pánková, Iveta
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issn
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number of pages
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