About: Staining of proteins for 2D SDS-PAGE using Coomassie Blue-speed versus sensitivity?     Goto   Sponge   NotDistinct   Permalink

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  • In this work, a new fast staining protocol recently introduced by Dong et al. (PLoS One 2011, 6, e22394) was compared to colloidal CBS. The number of detectable spots in 2D SDS-PAGE of identical blood plasma samples in repeated runs was chosen as a sensitivity criterion. Further, the influence of gel boiling on the subsequent protein identification by MS was investigated. In spite of its advantages, the staining protocol according to Dong et al. (PLoS One 2011, 6, e22394) seems to be less sensitive than colloidal Coomassie staining when the number of detected spots is the evaluating criterion. No obvious influence of gel boiling on the protein identification was observed.
  • In this work, a new fast staining protocol recently introduced by Dong et al. (PLoS One 2011, 6, e22394) was compared to colloidal CBS. The number of detectable spots in 2D SDS-PAGE of identical blood plasma samples in repeated runs was chosen as a sensitivity criterion. Further, the influence of gel boiling on the subsequent protein identification by MS was investigated. In spite of its advantages, the staining protocol according to Dong et al. (PLoS One 2011, 6, e22394) seems to be less sensitive than colloidal Coomassie staining when the number of detected spots is the evaluating criterion. No obvious influence of gel boiling on the protein identification was observed. (en)
Title
  • Staining of proteins for 2D SDS-PAGE using Coomassie Blue-speed versus sensitivity?
  • Staining of proteins for 2D SDS-PAGE using Coomassie Blue-speed versus sensitivity? (en)
skos:prefLabel
  • Staining of proteins for 2D SDS-PAGE using Coomassie Blue-speed versus sensitivity?
  • Staining of proteins for 2D SDS-PAGE using Coomassie Blue-speed versus sensitivity? (en)
skos:notation
  • RIV/00023736:_____/13:00010725!RIV14-GA0-00023736
http://linked.open...avai/riv/aktivita
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  • I, P(GBP205/12/G118)
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  • 13
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  • 107429
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  • RIV/00023736:_____/13:00010725
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  • Coomassie staining; fast staining protocol; Protein visualization (en)
http://linked.open.../riv/klicoveSlovo
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  • DE - Spolková republika Německo
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  • [49425636EB01]
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  • Electrophoresis
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  • 34
http://linked.open...iv/tvurceVysledku
  • Dyr, Jan Evangelista
  • Suttnar, Jiří
  • Májek, Pavel
  • Riedelová-Reicheltová, Zuzana
http://linked.open...ain/vavai/riv/wos
  • 000322112200018
issn
  • 0173-0835
number of pages
http://bibframe.org/vocab/doi
  • 10.1002/elps.201300087
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