About: Comparison of three single platform methods for CD34+ hematopoietic stem cell enumeration by flow cytometry

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About: Comparison of three single platform methods for CD34+ hematopoietic stem cell enumeration by flow cytometry Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=WOS&DestLinkType=FullRecord&UT=000298897400026
Description	In the present study, we compared three single platform methods for CD34+ hematopoietic stem cell (HSC) enumeration by flow cytometry. For this purpose, we analyzed the performance characteristics and results obtained from different HSC sources. Interlaboratory coefficients of variation (CV) for precision/reproducibility analysis varied from 4.0% to 6.7% / 6.7% to 9.2% for the low and 3.2% to 4.1% / 4.3% to 6.7%, respectively, for the high stem cell control. Correlation between methods ranged from 0.92% to 0.99%; Wilcoxon test showed no significant differences (p >0.05); Bland-Altman analysis confirmed good agreement between assays (mean bias ranging from -0.48 to 6.91). Our results demonstrate very good intralaboratory correlation and agreement between methods, confirm the major impact of single platform strategy for accurate and reproducible HSC enumeration and suggest that high interlaboratory variability could be influenced by incorrect performance of validated methods. In the present study, we compared three single platform methods for CD34+ hematopoietic stem cell (HSC) enumeration by flow cytometry. For this purpose, we analyzed the performance characteristics and results obtained from different HSC sources. Interlaboratory coefficients of variation (CV) for precision/reproducibility analysis varied from 4.0% to 6.7% / 6.7% to 9.2% for the low and 3.2% to 4.1% / 4.3% to 6.7%, respectively, for the high stem cell control. Correlation between methods ranged from 0.92% to 0.99%; Wilcoxon test showed no significant differences (p >0.05); Bland-Altman analysis confirmed good agreement between assays (mean bias ranging from -0.48 to 6.91). Our results demonstrate very good intralaboratory correlation and agreement between methods, confirm the major impact of single platform strategy for accurate and reproducible HSC enumeration and suggest that high interlaboratory variability could be influenced by incorrect performance of validated methods. (en)
Title	Comparison of three single platform methods for CD34+ hematopoietic stem cell enumeration by flow cytometry Comparison of three single platform methods for CD34+ hematopoietic stem cell enumeration by flow cytometry (en)
skos:prefLabel	Comparison of three single platform methods for CD34+ hematopoietic stem cell enumeration by flow cytometry Comparison of three single platform methods for CD34+ hematopoietic stem cell enumeration by flow cytometry (en)
skos:notation	RIV/00023736:_____/11:00009520!RIV13-MZ0-00023736
http://linked.open...avai/riv/aktivita	I V
http://linked.open...avai/riv/aktivity	I, V
http://linked.open...iv/cisloPeriodika	11-12
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Cetkovský, Petr Luxová, Alena Tkáčová, Vlasta Gašová, Zdenka Marinov, Iuri
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav hematologie a krevní transfúze
http://linked.open...dnocenehoVysledku	191290
http://linked.open...ai/riv/idVysledku	RIV/00023736:_____/11:00009520
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	flow cytometry; CD34+ cells; single platform methods (en)
http://linked.open.../riv/klicoveSlovo	CD34+ cells flow cytometry single platform methods
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[256872FED388]
http://linked.open...i/riv/nazevZdroje	Clinical laboratory
http://linked.open...in/vavai/riv/obor	FD
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	57
http://linked.open...iv/tvurceVysledku	Cetkovský, Petr Gašová, Zdenka Luxová, Alena Marinov, Iuri Pohlreich, D. Tkáčová, Vlasta
http://linked.open...ain/vavai/riv/wos	000298897400026
issn	1433-6510
number of pages	5 (xsd:int)

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