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Description
| - Zvýšená exprese genu Bmi-1 byla prokázána u různých typů rakoviny, což ukazuje, že Bmi-1 by mohl být potenciálním cílem pro jejich terapii. V této publikaci jsme studovali expresi Bmi-1 u chronické myeloidní leukemie (CML) a pomocí qRT-PCR jsme prokázali signifikantně zvýšenou hladinu jeho transkriptu u CML buněk. Pomocí cDNA čipů jsme po siRNA-tlumení exprese Bmi-1 dále detekovali deregulaci několika genů. Dále jsme testovali vliv siRNA-inhibice Bmi-1 genu na CML buněčné linie, avšak změnu v proliferaci buněk jsme nedetekovali. Přestože je Bmi-1 u CML zvýšeně exprimován a jeho produkce je spjata s transformačním procesem, hraje u CML pravděpodobně spíše sekundární úlohu (cs)
- It has been demonstrated that over-expression of Bmi-1 occurs in variety of cancers, which proposed Bmi-1 as a potential target for therapeutic intervention. In this study, we investigated the role of Bmi-1 in chronic myeloid leukemia (CML). Using qRT-PCR, we demonstrated significantly increased level of Bmi-1 transcript in CML cells. Using array analysis, we determined deregulation of several genes after Bmi-1 silencing. Further, we tested an impact of Bmi-1 siRNA on CML cell growth; however, there was no apparent change after Bmi-1 suppression. Despite Bmi-1 deregulation occurs in CML and its expression is connected to several oncogenic processes it seems to play rather secondary role in CML transformation
- It has been demonstrated that over-expression of Bmi-1 occurs in variety of cancers, which proposed Bmi-1 as a potential target for therapeutic intervention. In this study, we investigated the role of Bmi-1 in chronic myeloid leukemia (CML). Using qRT-PCR, we demonstrated significantly increased level of Bmi-1 transcript in CML cells. Using array analysis, we determined deregulation of several genes after Bmi-1 silencing. Further, we tested an impact of Bmi-1 siRNA on CML cell growth; however, there was no apparent change after Bmi-1 suppression. Despite Bmi-1 deregulation occurs in CML and its expression is connected to several oncogenic processes it seems to play rather secondary role in CML transformation (en)
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Title
| - Bmi-1 over-expression plays a secondary role in chronic myeloid leukemia transformation
- Bmi-1 over-expression plays a secondary role in chronic myeloid leukemia transformation (en)
- Zvýšená exprese Bmi-1 má sekundárním úlohu u chronické myeloidní leukemie (cs)
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skos:prefLabel
| - Bmi-1 over-expression plays a secondary role in chronic myeloid leukemia transformation
- Bmi-1 over-expression plays a secondary role in chronic myeloid leukemia transformation (en)
- Zvýšená exprese Bmi-1 má sekundárním úlohu u chronické myeloidní leukemie (cs)
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skos:notation
| - RIV/00023736:_____/07:00007112!RIV07-MZ0-00023736
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00023736:_____/07:00007112
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - CML; proliferation; siRNA; Bmi-1; expression arrays (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Klamová, Hana
- Brdička, Radim
- Merkerová, Michaela
- Bruchová, Hana
- Kráčmarová, Alžběta
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issn
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number of pages
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