About: Targeting of gene expression by siRNA in CML primary cells

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Za účelem vybrání nejvhodnějšího postupu vnášení siRNA molekul do primárních buněk chronické myeloidní leukemie (CML) byla testována škála fyzikálních a chemických non-vektorových transfekčích metod. Chemicky připravené siRNA proti několika genům byly transfekovány do buněk a účinnost transfekce byla určena pomocí qRT-PCR. Na základě našich výsledků bylo zjištěno, že nukleofekce (Amaxa) je jedinou účinnou metodou pro transfer siRNA do těžko transfekovatelných CML buněk (cs) We tested a variety of physical and chemical non-vector based transfection methods in order to evaluate which of them gave the most suitable transfer of siRNA into chronic myeloid leukemia (CML) primary cells. Chemically synthesized siRNAs against several genes were transfected into the cells and level of knockdown was determined by qRT-PCR. According to our results, nucleofection (Amaxa) appears to be the only suitable non-viral method for siRNA delivery into the hard-to-transfect CML primary cells We tested a variety of physical and chemical non-vector based transfection methods in order to evaluate which of them gave the most suitable transfer of siRNA into chronic myeloid leukemia (CML) primary cells. Chemically synthesized siRNAs against several genes were transfected into the cells and level of knockdown was determined by qRT-PCR. According to our results, nucleofection (Amaxa) appears to be the only suitable non-viral method for siRNA delivery into the hard-to-transfect CML primary cells (en)
Title	Targeting of gene expression by siRNA in CML primary cells Targeting of gene expression by siRNA in CML primary cells (en) Tlumení genové exprese pomocí siRNA u CML primárních buněk (cs)
skos:prefLabel	Targeting of gene expression by siRNA in CML primary cells Targeting of gene expression by siRNA in CML primary cells (en) Tlumení genové exprese pomocí siRNA u CML primárních buněk (cs)
skos:notation	RIV/00023736:_____/07:00007110!RIV07-MZ0-00023736
http://linked.open.../vavai/riv/strany	27;33
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(NR7989)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2007
http://linked.open...aciTvurceVysledku	Brdička, Radim Dostálová Merkerová, Michaela Votavová, Hana Klamová, Hana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav hematologie a krevní transfúze
http://linked.open...dnocenehoVysledku	454162
http://linked.open...ai/riv/idVysledku	RIV/00023736:_____/07:00007110
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	chemical transfection; CML; electroporation; nucleofection; siRNA; primary cells (en)
http://linked.open.../riv/klicoveSlovo	CML electroporation chemical transfection primary cells siRNA nucleofection
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[A02A16C392E7]
http://linked.open...i/riv/nazevZdroje	Molecular Biology Reports
http://linked.open...in/vavai/riv/obor	FD
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Targeted gene silencing using siRNAs in primary cell cultures of CML patients and leukemic cell lines (K562, MOLM-7, KU-812)
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	34
http://linked.open...iv/tvurceVysledku	Klamová, Hana Brdička, Radim Merkerová, Michaela Bruchová, Hana
issn	0301-4851
number of pages	12 (xsd:int)

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