About: Cloning and characterization of Sapp2p, the second aspartic proteinase isoenzyme from Candida parapsilosis

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About: Cloning and characterization of Sapp2p, the second aspartic proteinase isoenzyme from Candida parapsilosis Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Candida parapsilosis obsahuje 3 geny kódující sekretované aspartátové proteázy. Prekursor proteázy Sapp2p byl exprimován v E. coli a purifikován. Autoaktivace Sapp2p byla málo účinná a vedla ke vzniku proteinu prodlouženého na N-konci o 8 aminokyselin. K aktivaci v očekávaném místě došlo účinkem trypsinu nebo membránové proteázy Kex2 z C. parapsilosis. Maturní Sapp2p byla proteolyticky aktivní a její substrátová specifita se lišila od specifity Sapp1p. (cs) Candida parapsilosis possesses 3 genes encoding secreted aspartic proteinases. The Sapp2p precursor was expressed in E. coli and purified. Autoactivation of pro-Sapp2p was inefficient and resulted in a protein extended by 8 amino acids at the N-terminus. The correct promature junction was cleaved by trypsin or by a membrane-bound Kex2-like proteinase from C. parapsilosis. The mature Sapp2p was proteolytically active and its substrate specificity differed from that of Sapp1p. Candida parapsilosis possesses 3 genes encoding secreted aspartic proteinases. The Sapp2p precursor was expressed in E. coli and purified. Autoactivation of pro-Sapp2p was inefficient and resulted in a protein extended by 8 amino acids at the N-terminus. The correct promature junction was cleaved by trypsin or by a membrane-bound Kex2-like proteinase from C. parapsilosis. The mature Sapp2p was proteolytically active and its substrate specificity differed from that of Sapp1p. (en)
Title	Cloning and characterization of Sapp2p, the second aspartic proteinase isoenzyme from Candida parapsilosis Klonování a charakterizace Sapp2p, druhého izoenzymu aspartátové proteiasy z Candida parapsilosis (cs) Cloning and characterization of Sapp2p, the second aspartic proteinase isoenzyme from Candida parapsilosis (en)
skos:prefLabel	Cloning and characterization of Sapp2p, the second aspartic proteinase isoenzyme from Candida parapsilosis Klonování a charakterizace Sapp2p, druhého izoenzymu aspartátové proteiasy z Candida parapsilosis (cs) Cloning and characterization of Sapp2p, the second aspartic proteinase isoenzyme from Candida parapsilosis (en)
skos:notation	RIV/00023736:_____/06:00006659!RIV09-MZ0-00023736
http://linked.open...avai/riv/aktivita	P V Z
http://linked.open...avai/riv/aktivity	P(GA203/05/0038), P(GA303/04/0432), P(LC531), V, Z(AV0Z40550506)
http://linked.open...iv/cisloPeriodika	7
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Dostálová Merkerová, Michaela
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav hematologie a krevní transfúze
http://linked.open...dnocenehoVysledku	468897
http://linked.open...ai/riv/idVysledku	RIV/00023736:_____/06:00006659
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Candida parapsilosis; aspartic proteinase (en)
http://linked.open.../riv/klicoveSlovo	Candida parapsilosis aspartic proteinase
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[85B39138B0AB]
http://linked.open...i/riv/nazevZdroje	FEMS yeast research
http://linked.open...in/vavai/riv/obor	FD
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Centre on molecular biology and physiology of yeast communities Study of the role of different isoenzymes of aspartic proteases in the virulence of Candida spp. Mechanism of activation of the proteinases secreted by the pathogenic yeast Candida parapsilosis
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	6
http://linked.open...iv/tvurceVysledku	Dostál, J. Pichová, I. Merkerová, Michaela Hrušková-Heidingsfeldová, O. Hradilek, M.
http://linked.open...ain/vavai/riv/wos	000241200400009
http://linked.open...n/vavai/riv/zamer	Regulace biologických procesů: Chemické modulátory vybraných systémů významných pro medicínu a zemědělství
issn	1567-1356
number of pages	9 (xsd:int)

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