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| Description
| - Unconjugated bilirubin (UCB) exhibits potent antioxidant and cytoprotective properties, but causes apoptosis and cytotoxicity at pathologically elevated concentrations. Accurate measurement of UCB concentrations in cells, fluids and tissues is needed to evaluate its role in redox regulation, prevention of atherosclerotic and malignant diseases, and bilirubin encephalopathy. In the present study, we developed and validated a highly sensitive method for tissue UCB determinations. UCB was extracted from rat organs with chloroform/methanol/hexane at pH 6.2 and then partitioned into a minute volume of alkaline buffer that was subjected to HPLC using an octyl reverse phase (RP) column. Addition of mesobilirubin as an internal standard corrected for losses of UCB during extraction. Recoveries averaged 75+/-5%. The detection limit was 10pmol UCB/g wet tissue. Variance was +/-2.5%. When used to measure UCB concentrations in tissues of jaundiced Gunn rats, this procedure yielded UCB levels directly comparable t
- Unconjugated bilirubin (UCB) exhibits potent antioxidant and cytoprotective properties, but causes apoptosis and cytotoxicity at pathologically elevated concentrations. Accurate measurement of UCB concentrations in cells, fluids and tissues is needed to evaluate its role in redox regulation, prevention of atherosclerotic and malignant diseases, and bilirubin encephalopathy. In the present study, we developed and validated a highly sensitive method for tissue UCB determinations. UCB was extracted from rat organs with chloroform/methanol/hexane at pH 6.2 and then partitioned into a minute volume of alkaline buffer that was subjected to HPLC using an octyl reverse phase (RP) column. Addition of mesobilirubin as an internal standard corrected for losses of UCB during extraction. Recoveries averaged 75+/-5%. The detection limit was 10pmol UCB/g wet tissue. Variance was +/-2.5%. When used to measure UCB concentrations in tissues of jaundiced Gunn rats, this procedure yielded UCB levels directly comparable t (en)
- Nekonjugovaný bilirubin má antioxidační a cytoprotektivní účinky, ve vysokých koncentracích však vyvolává apoptózu a je cytotoxický. Přesné měření bilirubinu ve tkáních je nezbytné ke studiu jeho úlohy v redoxních regulacích, prevenci aterosklerózy a malignit. V naší studii jsme vyvinuli a validovali vysoce citlivou chromatografickou metodu detekce tkáňového bilirubinu. Výsledky měření ve tkáních Gunnových potkanů byly srovnatelné s publikovanými hodnotami naměřenými jinými technikami. (cs)
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| Title
| - Highly sensitive method for quantitative determination of bilirubin in biological fluids and tissues
- Highly sensitive method for quantitative determination of bilirubin in biological fluids and tissues (en)
- Vysoce citlivá metoda pro stanovení bilirubinu v biologických tekutinách a tkáních (cs)
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| skos:prefLabel
| - Highly sensitive method for quantitative determination of bilirubin in biological fluids and tissues
- Highly sensitive method for quantitative determination of bilirubin in biological fluids and tissues (en)
- Vysoce citlivá metoda pro stanovení bilirubinu v biologických tekutinách a tkáních (cs)
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| skos:notation
| - RIV/00023001:_____/08:00001760!RIV09-MZ0-00023001
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(ME 849), Z(MZ0IKEM2005)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00023001:_____/08:00001760
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - bilirubin; Gunn rats; HPLC; method of determination; tissue bilirubin (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Journal of Chromatography B : Analytical technologies in the biomedical and life sciences
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Baláž, Peter
- Jirsa, Milan
- Muchová, L.
- Leníček, M.
- Vítek, L.
- Zadinová, M.
- Kudla, Michal
- Wong, R. J.
- Zelenka, J.
- Ostrow, J. D.
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| http://linked.open...ain/vavai/riv/wos
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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