About: Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5α in Bioaerosols     Goto   Sponge   NotDistinct   Permalink

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Description
  • The portable detector ImmunoSMART was combined with the cyclone air sampler to detect the model bioagent E. coli DH5α in bioaerosols. The selected capture antibody was immobilized on gold electrodes, antibody-peroxidase conjugate served as a tracer. In buffer, the amperometric immunosensor measured 103 to 108 CFU/mL within 30 min. Detection in air was realized in a closed aerosol chamber. Automated dissemination of the cells, sampling and measurement allowed remote testing of the cyclone/immunodetector. The level of 150 CFU/L in air was clearly indicated (20 min analysis including 5 min sampling) and independently confirmed using the slit sampler and agar cultivation. The results seem promising for further extended tests in both aerosol chamber and field trial conditions.
  • The portable detector ImmunoSMART was combined with the cyclone air sampler to detect the model bioagent E. coli DH5α in bioaerosols. The selected capture antibody was immobilized on gold electrodes, antibody-peroxidase conjugate served as a tracer. In buffer, the amperometric immunosensor measured 103 to 108 CFU/mL within 30 min. Detection in air was realized in a closed aerosol chamber. Automated dissemination of the cells, sampling and measurement allowed remote testing of the cyclone/immunodetector. The level of 150 CFU/L in air was clearly indicated (20 min analysis including 5 min sampling) and independently confirmed using the slit sampler and agar cultivation. The results seem promising for further extended tests in both aerosol chamber and field trial conditions. (en)
Title
  • Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5α in Bioaerosols
  • Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5α in Bioaerosols (en)
skos:prefLabel
  • Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5α in Bioaerosols
  • Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5α in Bioaerosols (en)
skos:notation
  • RIV/00000493:G41__/11:#0000388!RIV12-MO0-G41_____
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(OVVTUO2008001)
http://linked.open...iv/cisloPeriodika
  • 3
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 197034
http://linked.open...ai/riv/idVysledku
  • RIV/00000493:G41__/11:#0000388
http://linked.open...riv/jazykVysledku
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  • Bioagents; Sandwich immunoassay; Biosensors; Remote sensing; Aerosol chamber (en)
http://linked.open.../riv/klicoveSlovo
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  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [1295AE0E9F10]
http://linked.open...i/riv/nazevZdroje
  • Electroanalysis
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
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http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 24
http://linked.open...iv/tvurceVysledku
  • Skládal, Petr
  • Švábenská, Eva
issn
  • 1040-0397
number of pages
http://localhost/t...ganizacniJednotka
  • G41
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