About: Ms1, a novel sRNA interacting with the RNA polymerase core in mycobacteria

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About: Ms1, a novel sRNA interacting with the RNA polymerase core in mycobacteria Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Small RNAs (sRNAs) are molecules essential for a number of regulatory processes in the bacterial cell. Here we characterize Ms1, a sRNA that is highly expressed in Mycobacterium smegmatis during stationary phase of growth. By glycerol gradient ultracentrifugation, RNA binding assay, and RNA co-immunoprecipitation, we show that Ms1 interacts with the RNA polymerase (RNAP) core that is free of the primary sigma factor (sigma(A)) or any other sigma factor. This contrasts with the situation in most other species where it is 6S RNA that interacts with RNAP and this interaction requires the presence of sigma(A). The difference in the interaction of the two types of sR-NAs (Ms1 or 6S RNA) with RNAP possibly reflects the difference in the composition of the transcriptional machinery between mycobacteria and other species. Unlike Escherichia coli, stationary phase M. smegmatis cells contain relatively few RNAP molecules in complex with sigma(A). Thus, Ms1 represents a novel type of small RNAs interacting with RNAP. Small RNAs (sRNAs) are molecules essential for a number of regulatory processes in the bacterial cell. Here we characterize Ms1, a sRNA that is highly expressed in Mycobacterium smegmatis during stationary phase of growth. By glycerol gradient ultracentrifugation, RNA binding assay, and RNA co-immunoprecipitation, we show that Ms1 interacts with the RNA polymerase (RNAP) core that is free of the primary sigma factor (sigma(A)) or any other sigma factor. This contrasts with the situation in most other species where it is 6S RNA that interacts with RNAP and this interaction requires the presence of sigma(A). The difference in the interaction of the two types of sR-NAs (Ms1 or 6S RNA) with RNAP possibly reflects the difference in the composition of the transcriptional machinery between mycobacteria and other species. Unlike Escherichia coli, stationary phase M. smegmatis cells contain relatively few RNAP molecules in complex with sigma(A). Thus, Ms1 represents a novel type of small RNAs interacting with RNAP. (en)
Title	Ms1, a novel sRNA interacting with the RNA polymerase core in mycobacteria Ms1, a novel sRNA interacting with the RNA polymerase core in mycobacteria (en)
skos:prefLabel	Ms1, a novel sRNA interacting with the RNA polymerase core in mycobacteria Ms1, a novel sRNA interacting with the RNA polymerase core in mycobacteria (en)
skos:notation	RIV/61388971:_____/14:00439950!RIV15-GA0-61388971
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GBP305/12/G034), P(GP13-27150P)
http://linked.open...iv/cisloPeriodika	18
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Pospíšil, Jiří Šiková, Michaela Jirát-Matějčková, Jitka Halada, Petr Hnilicová, Jarmila Krásný, Libor Pánek, Josef
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	30906
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/14:00439950
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	ESCHERICHIA-COLI; 6S RNA; NONCODING RNA (en)
http://linked.open.../riv/klicoveSlovo	6S RNA ESCHERICHIA-COLI NONCODING RNA
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[859D55596A31]
http://linked.open...i/riv/nazevZdroje	Nucleic Acids Research
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	7 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Centre for RNA Biology Molekulární mechanismy přežití bakterií v nepříznivých podmínkách
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	42
http://linked.open...iv/tvurceVysledku	Halada, Petr Krásný, Libor Pospíšil, Jiří Hnilicová, Jarmila Pánek, Josef Jirát-Matějčková, Jitka Šiková, Michaela
http://linked.open...ain/vavai/riv/wos	000347687100047
issn	0305-1048
number of pages	14 (xsd:int)

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