About: Preparation of short cytosine-modified oligonucleotides by nicking enzyme amplification reaction     Goto   Sponge   NotDistinct   Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

AttributesValues
rdf:type
Description
  • A method for enzymatic production of short (10-20 nt) cytosine-modified oligonucleotides was developed by nicking enzyme amplification reaction using Vent(exo-) polymerase, Nt.BstNBI nicking endonuclease and 5-substituted dCTP derivatives. The methodology including isolation was scaled up to nanomolar amounts and was proved to be suitable for production of diverse base-modified short single-stranded oligonucleotides (inaccessible by other enzymatic methods) that are of potential interest as labelled primers or functionalized aptamers.
  • A method for enzymatic production of short (10-20 nt) cytosine-modified oligonucleotides was developed by nicking enzyme amplification reaction using Vent(exo-) polymerase, Nt.BstNBI nicking endonuclease and 5-substituted dCTP derivatives. The methodology including isolation was scaled up to nanomolar amounts and was proved to be suitable for production of diverse base-modified short single-stranded oligonucleotides (inaccessible by other enzymatic methods) that are of potential interest as labelled primers or functionalized aptamers. (en)
Title
  • Preparation of short cytosine-modified oligonucleotides by nicking enzyme amplification reaction
  • Preparation of short cytosine-modified oligonucleotides by nicking enzyme amplification reaction (en)
skos:prefLabel
  • Preparation of short cytosine-modified oligonucleotides by nicking enzyme amplification reaction
  • Preparation of short cytosine-modified oligonucleotides by nicking enzyme amplification reaction (en)
skos:notation
  • RIV/61388963:_____/12:00377953!RIV13-AV0-61388963
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • I, P(GA203/09/0317)
http://linked.open...iv/cisloPeriodika
  • 55
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 161362
http://linked.open...ai/riv/idVysledku
  • RIV/61388963:_____/12:00377953
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • cross-coupling reactions; nucleoside triphosphates; functionalized DNA; restriction endonucleases; polymerase incorporation (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [8E3AE809B893]
http://linked.open...i/riv/nazevZdroje
  • Chemical Communications
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 48
http://linked.open...iv/tvurceVysledku
  • Hocek, Michal
  • Ménová, Petra
http://linked.open...ain/vavai/riv/wos
  • 000305255300010
issn
  • 1359-7345
number of pages
http://bibframe.org/vocab/doi
  • 10.1039/c2cc32930a
Faceted Search & Find service v1.16.118 as of Jun 21 2024


Alternative Linked Data Documents: ODE     Content Formats:   [cxml] [csv]     RDF   [text] [turtle] [ld+json] [rdf+json] [rdf+xml]     ODATA   [atom+xml] [odata+json]     Microdata   [microdata+json] [html]    About   
This material is Open Knowledge   W3C Semantic Web Technology [RDF Data] Valid XHTML + RDFa
OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 112 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software