About: Isolation and Characterization of Protoplasts and their Utilization for Model Membrane

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Protoplasts are prepared form plant cells by removing their cell walls, which are usually destroyed mechanically or by enzymes. Protoplasts exhibit the spherical shape after removing of their cell walls. They can be isolated from a range of plant tissues: leaves, stems, roots, anthers, and even pollen. Plasmatic membranes surrounding protoplasts can be used as a source of model membranes. Therefore, some parts of plasmatic membranes or subcellular organelle membranes, which contain specific transport systems, can be isolated and integrated to model phospholipid membranes. State of such model membranes can be characterized by electrochemical impedance spectrometry (EIS) and the transported species can be determined with the use of voltammetric methods. Protoplasts are prepared form plant cells by removing their cell walls, which are usually destroyed mechanically or by enzymes. Protoplasts exhibit the spherical shape after removing of their cell walls. They can be isolated from a range of plant tissues: leaves, stems, roots, anthers, and even pollen. Plasmatic membranes surrounding protoplasts can be used as a source of model membranes. Therefore, some parts of plasmatic membranes or subcellular organelle membranes, which contain specific transport systems, can be isolated and integrated to model phospholipid membranes. State of such model membranes can be characterized by electrochemical impedance spectrometry (EIS) and the transported species can be determined with the use of voltammetric methods. (en)
Title	Isolation and Characterization of Protoplasts and their Utilization for Model Membrane Isolation and Characterization of Protoplasts and their Utilization for Model Membrane (en)
skos:prefLabel	Isolation and Characterization of Protoplasts and their Utilization for Model Membrane Isolation and Characterization of Protoplasts and their Utilization for Model Membrane (en)
skos:notation	RIV/61388955:_____/14:00428764!RIV15-GA0-61388955
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GAP208/12/1645)
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Langmaier, Jan Heyrovský, Michael Šestáková, Ivana Navrátil, Tomáš Nováková, Kateřina
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav fyzikální chemie J. Heyrovského AV ČR, v.v.i.
http://linked.open...dnocenehoVysledku	22950
http://linked.open...ai/riv/idVysledku	RIV/61388955:_____/14:00428764
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Protoplasts; Cells; Model membrane (en)
http://linked.open.../riv/klicoveSlovo	Model membrane Protoplasts Cells
http://linked.open...ontrolniKodProRIV	[9894C6F631EA]
http://linked.open...v/mistoKonaniAkce	Jetřichovice
http://linked.open...i/riv/mistoVydani	Ústí nad Labem
http://linked.open...i/riv/nazevZdroje	Sborník přednášek mezinárodní odborné konference XXXIV. Moderní Elektrochemické Metody
http://linked.open...in/vavai/riv/obor	CG
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Aspects of hazardous metals transfer across biomembranes
http://linked.open...UplatneniVysledku	2014
http://linked.open...iv/tvurceVysledku	Langmaier, Jan Navrátil, Tomáš Nováková, Kateřina Šestáková, Ivana Heyrovský, Michael Vodičková, H. Zámečníková, B.
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2014-05-19 (xsd:date)
number of pages	4 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Best Servis
https://schema.org/isbn	978-80-905221-2-1

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