About: Extracellular phosphatase activity of natural plankton studied with ELF97 phosphate: fluorescence quantification and labelling kinetics.

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About: Extracellular phosphatase activity of natural plankton studied with ELF97 phosphate: fluorescence quantification and labelling kinetics. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	ELF97 phosphate is a phosphatase substrate which produces ELFA, a fluorescent water-insoluble product, upon hydrolysis. We studied the kinetics of ELFA precipitation in acidic Plešné Lake (pH about 5; high phosphatase activity) and eutrophic Římov reservoir (pH 7-10; moderate phosphatase activity). Relative fluorescence ELFA precipitates was quantified with image analysis. Time-courses of ELFA formation exhibited lag periods followed by finite periods of linear increase. In Plešné Lake, lag-times were 1-18 min and rates of increase in ELFA fluorescence higher (by about 2 orders of magnitude) than in Římov reservoir (lag-times 30-200 min). Linear regression of seasonal data on rates of increase in ELFA fluorescence from image cytometry and spectrofluorometry (r (2) = 0.65, n = 10) allowed for calibration of image cytometry. Preliminary measurements of extracellular phosphatase activities of several algae resulted in rates 10-2260 fmol cell(-1) h(-1). ELF97 phosphate is a phosphatase substrate which produces ELFA, a fluorescent water-insoluble product, upon hydrolysis. We studied the kinetics of ELFA precipitation in acidic Plešné Lake (pH about 5; high phosphatase activity) and eutrophic Římov reservoir (pH 7-10; moderate phosphatase activity). Relative fluorescence ELFA precipitates was quantified with image analysis. Time-courses of ELFA formation exhibited lag periods followed by finite periods of linear increase. In Plešné Lake, lag-times were 1-18 min and rates of increase in ELFA fluorescence higher (by about 2 orders of magnitude) than in Římov reservoir (lag-times 30-200 min). Linear regression of seasonal data on rates of increase in ELFA fluorescence from image cytometry and spectrofluorometry (r (2) = 0.65, n = 10) allowed for calibration of image cytometry. Preliminary measurements of extracellular phosphatase activities of several algae resulted in rates 10-2260 fmol cell(-1) h(-1). (en)
Title	Extracellular phosphatase activity of natural plankton studied with ELF97 phosphate: fluorescence quantification and labelling kinetics. Extracellular phosphatase activity of natural plankton studied with ELF97 phosphate: fluorescence quantification and labelling kinetics. (en)
skos:prefLabel	Extracellular phosphatase activity of natural plankton studied with ELF97 phosphate: fluorescence quantification and labelling kinetics. Extracellular phosphatase activity of natural plankton studied with ELF97 phosphate: fluorescence quantification and labelling kinetics. (en)
skos:notation	RIV/60077387:_____/03:62033016!RIV/2004/AV0/A62004/N
http://linked.open.../vavai/riv/strany	462;472
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA206/00/0063), P(IAA6017202), P(IBS6017004), Z(AV0Z6017912)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Nedoma, Jiří Šimek, Karel Ševců, Alena Vrba, Jaroslav Komárková, Jaroslava
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Hydrobiologický ústav AV ČR
http://linked.open...dnocenehoVysledku	606951
http://linked.open...ai/riv/idVysledku	RIV/60077387:_____/03:62033016
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	phosphorus; phytoplankton; lakes (en)
http://linked.open.../riv/klicoveSlovo	phosphorus lakes phytoplankton - chlorophyll-a - running waters - long-term changes - nuclear power plant Temelín
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[B788155F8988]
http://linked.open...i/riv/nazevZdroje	Environmental Microbiology
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Biogeochemical cycles of nutrients in mountain catchment-lake ecosystems: Anthropogenic impacts and possibilities of recovery Detection, localisation and quantification of extracellular enzymatic activities on cellular and population level in natural lake seston Water quality control in reservoirs
http://linked.open...UplatneniVysledku	2003
http://linked.open...v/svazekPeriodika	5
http://linked.open...iv/tvurceVysledku	Komárková, Jaroslava Šimek, Karel Nedoma, Jiří Vrba, Jaroslav Štrojsová, Alena
http://linked.open...n/vavai/riv/zamer	Biotické interakce v pelagické zóně lenitických ekosystémů, údolních nádrží a jezer různé trofie v podmínkách výrazného snížení emisí v postkomunistické Evropě
issn	1462-2912
number of pages	11 (xsd:int)

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