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| Description
| - Dosud není známa funkce 2’-O-ribozové metylace v čepičce typu 1 u žádného eukaryota, ačkoli se jedná o vysoce konzervovanou medifikaci. Díky sestřihu formou trans z polycistronických prekurzorů prostřednictvím spliced leader (SL) dochází u kinetoplastidových bičíkovců k přenosu hypermetylované čepičky typu 4 na všechny jaderné poslíčkové RNA. V této práci jsme charakterizovali jako první eukaryotickou čepičkovou 2’-O-ribozovou metyltransferázu TbMtr1, která je členem konzervované rodiny virových a eukaryotických enzymů. Knockdownové a knockoutové linie Trypanosoma brucei pro gen TbMTr1 rostou normálně, ačkoli se u nich nachází ztráta 2’-O-ribozové metylace na čepičce typu 1 u SL a U1 malých jaderných RNA. U knockoutové linie pro TbMTr1 došlo k akumulaci substrátu čepičky O, ačkoli je sestřihaná poslíčková RNA modifikována v pozici 4 a postrádá metylaci 2’-O-ribozy v pozici 1. (cs)
- The function of cap 1 2'-O-ribose methylation is not known in any eukaryotic system, although it is a conserved modification. In kinetoplastid flagellates trans-splicing of spliced leader (SL) to polycistronic precursors conveys the hypermethylated cap 4 to all nuclear messenger RNAs. We report the first eukaryotic cap 1 2'-O-ribose methyltransferase, TbMTr1, a member of a conserved family of viral and eukaryotic enzymes. Recombinant TbMTr1 methylates the ribose of the first nucleotide of a m7G-capped substrate. Knockdowns and knockouts of TbMTr1 in Trypanosoma brucei grow normally, while displaying loss of 2'-O-ribose methylation at cap 1 on SL and U1 snRNA. TbMTr1 knockouts have an accumulation of cap 0 substrate, however spliced messenger RNA is modified at position 4 and lacks 2'-O-ribose methylation at position 1. Accumulation of 3'-extended SL indicates a delay in processing, and suggests a synergistic role for cap 1 in maturation of substrate SL RNA.
- The function of cap 1 2'-O-ribose methylation is not known in any eukaryotic system, although it is a conserved modification. In kinetoplastid flagellates trans-splicing of spliced leader (SL) to polycistronic precursors conveys the hypermethylated cap 4 to all nuclear messenger RNAs. We report the first eukaryotic cap 1 2'-O-ribose methyltransferase, TbMTr1, a member of a conserved family of viral and eukaryotic enzymes. Recombinant TbMTr1 methylates the ribose of the first nucleotide of a m7G-capped substrate. Knockdowns and knockouts of TbMTr1 in Trypanosoma brucei grow normally, while displaying loss of 2'-O-ribose methylation at cap 1 on SL and U1 snRNA. TbMTr1 knockouts have an accumulation of cap 0 substrate, however spliced messenger RNA is modified at position 4 and lacks 2'-O-ribose methylation at position 1. Accumulation of 3'-extended SL indicates a delay in processing, and suggests a synergistic role for cap 1 in maturation of substrate SL RNA. (en)
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| Title
| - The 2’-O-ribose methyltransferase for cap 1 of spliced leader RNA and U1 small nuclear RNA in Trypanosoma brucei
- 2’-O-ribose methyltransferáza pro čepičku 1 spliced leader RNA a U1 malé jaderné RNA v Trypanosoma brucei (cs)
- The 2’-O-ribose methyltransferase for cap 1 of spliced leader RNA and U1 small nuclear RNA in Trypanosoma brucei (en)
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| skos:prefLabel
| - The 2’-O-ribose methyltransferase for cap 1 of spliced leader RNA and U1 small nuclear RNA in Trypanosoma brucei
- 2’-O-ribose methyltransferáza pro čepičku 1 spliced leader RNA a U1 malé jaderné RNA v Trypanosoma brucei (cs)
- The 2’-O-ribose methyltransferase for cap 1 of spliced leader RNA and U1 small nuclear RNA in Trypanosoma brucei (en)
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| skos:notation
| - RIV/60077344:_____/07:00091123!RIV08-AV0-60077344
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(2B06129), P(LC07032), S, Z(AV0Z60220518)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/60077344:_____/07:00091123
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - methylation; Trypanosoma brucei; methyltransferase; RNA interference (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Molecular and Cellular Biology
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Lukeš, Julius
- Foldynová-Trantírková, Silvie
- Campbell, D. A.
- Sturm, N. R.
- Zeiner, G. M.
- Bujnicki, J. M.
- Mittra, B.
- Zamudio, J. R.
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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