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| rdfs:seeAlso
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| Description
| - The aim of this study was to determine relationship between parameters of spermatozoa motility (total motility - TM and progressive movement - PM) and viability of bull frozen-thawed spermatozoa (dead spermatozoa ratio, apoptotic spermatozoa ratio and plasma membrane integrity). Motility parameters were evaluated using computer-assisted semen analysis (CASA). Parameters of spermatozoa viability were analysed using fluorescent dyes PNA-FITC (plasma membrane), Yo-Pro-1 and propidium iodide (PI). All bulls (n=6) were divided into two groups. First group (n=3) A – better bulls with total motility after thawing over 40% and the second group (n=3) B – with total motility lower than 40%. It was observed significantly (P<0.001) higher TM and PM in group A. No significant differences in velocity parameters and ALH between the group A and B were detected. Occurrence of spermatozoa with disrupted membranes, dead/necrotic spermatozoa and apoptotic spermatozoa was significantly lower in the group A. Bulls in the group A showed significantly higher cleavage rate of embryos. These motility and viability characteristics are associated with a higher embryo cleavage rate in in vitro fertilization test.
- The aim of this study was to determine relationship between parameters of spermatozoa motility (total motility - TM and progressive movement - PM) and viability of bull frozen-thawed spermatozoa (dead spermatozoa ratio, apoptotic spermatozoa ratio and plasma membrane integrity). Motility parameters were evaluated using computer-assisted semen analysis (CASA). Parameters of spermatozoa viability were analysed using fluorescent dyes PNA-FITC (plasma membrane), Yo-Pro-1 and propidium iodide (PI). All bulls (n=6) were divided into two groups. First group (n=3) A – better bulls with total motility after thawing over 40% and the second group (n=3) B – with total motility lower than 40%. It was observed significantly (P<0.001) higher TM and PM in group A. No significant differences in velocity parameters and ALH between the group A and B were detected. Occurrence of spermatozoa with disrupted membranes, dead/necrotic spermatozoa and apoptotic spermatozoa was significantly lower in the group A. Bulls in the group A showed significantly higher cleavage rate of embryos. These motility and viability characteristics are associated with a higher embryo cleavage rate in in vitro fertilization test. (en)
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| Title
| - Relationship between motility and viability parameters of frozen-thawed bull spermatozoa
- Relationship between motility and viability parameters of frozen-thawed bull spermatozoa (en)
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| skos:prefLabel
| - Relationship between motility and viability parameters of frozen-thawed bull spermatozoa
- Relationship between motility and viability parameters of frozen-thawed bull spermatozoa (en)
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| skos:notation
| - RIV/49608851:_____/13:#0000592!RIV14-MZE-49608851
|
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| |
| http://linked.open...iv/cisloPeriodika
| - January 2013 - Special Issue 1
|
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/49608851:_____/13:#0000592
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - frozen-thawed spermatozoa; viability; motility (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| |
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| - The journal of microbiology, biotechnology and food sciences
|
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...iv/tvurceVysledku
| - Kubovičová, Elena
- Makarevič, Alexander
- Olexíková, Lucia
- Špaleková, Eliška
- Pivko, Juraj
|
| issn
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| number of pages
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