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Description
  • Recently, we have described a high-resolution cytometry (HRCM) technique (Cytometry 36:279-293) as a reasonable compromise between flow cytometry (FCM) and laser scanning cytometry (LSCM) on one hand and confocal laser scanning microscopy (CLSM) on the other hand. Whereas FCM/LSCM and CLSM enable measuring of either large number of cells with low resolution or small number of cells with high resolution, respectively, HRCM can analyze quantities comparable to FCM/LSCM measurements (using overnight acquisition) with an accuracy comparable to CLSM. Very recently we have built a second HRCM instrument (see picture at http://www.fi.muni.cz/lom) which enables switching between conventional and confocal modes. The confocal mode is realized using a 5% Nipkow disk optimized for fluorescence imaging. It offers a better resolution but at a lower speed. The user can choose which fluorochromes are imaged in confocal mode. Thus, the system can be configured for a variety of imaging modes ranging from quick 2-D anal
  • Recently, we have described a high-resolution cytometry (HRCM) technique (Cytometry 36:279-293) as a reasonable compromise between flow cytometry (FCM) and laser scanning cytometry (LSCM) on one hand and confocal laser scanning microscopy (CLSM) on the other hand. Whereas FCM/LSCM and CLSM enable measuring of either large number of cells with low resolution or small number of cells with high resolution, respectively, HRCM can analyze quantities comparable to FCM/LSCM measurements (using overnight acquisition) with an accuracy comparable to CLSM. Very recently we have built a second HRCM instrument (see picture at http://www.fi.muni.cz/lom) which enables switching between conventional and confocal modes. The confocal mode is realized using a 5% Nipkow disk optimized for fluorescence imaging. It offers a better resolution but at a lower speed. The user can choose which fluorochromes are imaged in confocal mode. Thus, the system can be configured for a variety of imaging modes ranging from quick 2-D anal (en)
  • Recently, we have described a high-resolution cytometry (HRCM) technique (Cytometry 36:279-293) as a reasonable compromise between flow cytometry (FCM) and laser scanning cytometry (LSCM) on one hand and confocal laser scanning microscopy (CLSM) on the other hand. Whereas FCM/LSCM and CLSM enable measuring of either large number of cells with low resolution or small number of cells with high resolution, respectively, HRCM can analyze quantities comparable to FCM/LSCM measurements (using overnight acquisition) with an accuracy comparable to CLSM. Very recently we have built a second HRCM instrument (see picture at http://www.fi.muni.cz/lom) which enables switching between conventional and confocal modes. The confocal mode is realized using a 5% Nipkow disk optimized for fluorescence imaging. It offers a better resolution but at a lower speed. The user can choose which fluorochromes are imaged in confocal mode. Thus, the system can be configured for a variety of imaging modes ranging from quick 2-D anal (cs)
Title
  • Advances in high-resolution cytometry of FISH dots in interphase cell nuclei
  • Advances in high-resolution cytometry of FISH dots in interphase cell nuclei (en)
  • Advances in high-resolution cytometry of FISH dots in interphase cell nuclei (cs)
skos:prefLabel
  • Advances in high-resolution cytometry of FISH dots in interphase cell nuclei
  • Advances in high-resolution cytometry of FISH dots in interphase cell nuclei (en)
  • Advances in high-resolution cytometry of FISH dots in interphase cell nuclei (cs)
skos:notation
  • RIV/00216224:14330/00:00005455!RIV09-MSM-14330___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM 143300002)
http://linked.open...iv/cisloPeriodika
  • 10
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 704013
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14330/00:00005455
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • automated microscopy; image analysis; high-resolution cytometry; fluorescence in situ hybridization; interphase nuclei; 3-D analysis (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [20E774A8CD1B]
http://linked.open...i/riv/nazevZdroje
  • Cytometry
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • Supplement
http://linked.open...iv/tvurceVysledku
  • Bártová, Eva
  • Kozubek, Stanislav
  • Lukášová, Emilie
  • Matula, Pavel
  • Kozubek, Michal
  • Matula, Petr
  • Koutná, Irena
  • Gajdušková, Pavla
  • Skalníková, Magdalena
  • Gaňová, Alena
http://linked.open...n/vavai/riv/zamer
issn
  • 0196-4763
number of pages
http://localhost/t...ganizacniJednotka
  • 14330
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