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Description
  • A new method for specific determination of dithiothreitol (DTT) using micellar electrokinetic chromatography and on-column reaction with reactive disulfide - 2,2-dipyridyldisulfide is described. DTT is in this reaction quantitatively transformed into a mixed disulfide concomitantly with formation of equimolar amount of the 2-thiopyridone that is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm. The concentration of DTT is thus estimated indirectly from the result of 2-thiopyridone determination. The linear detection range for concentration versus peak area for the assay is from 0.05 to 2.5 mM (correlation coefficient 0.993) with a detection limit of 2.5 mM. The inter-day reproducibility of the peak area was 1.35 % and the inter-day reproducibility of the migration time 0.56 %. The method can be applied for DTT monitoring both in chemical and biological systems.
  • A new method for specific determination of dithiothreitol (DTT) using micellar electrokinetic chromatography and on-column reaction with reactive disulfide - 2,2-dipyridyldisulfide is described. DTT is in this reaction quantitatively transformed into a mixed disulfide concomitantly with formation of equimolar amount of the 2-thiopyridone that is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm. The concentration of DTT is thus estimated indirectly from the result of 2-thiopyridone determination. The linear detection range for concentration versus peak area for the assay is from 0.05 to 2.5 mM (correlation coefficient 0.993) with a detection limit of 2.5 mM. The inter-day reproducibility of the peak area was 1.35 % and the inter-day reproducibility of the migration time 0.56 %. The method can be applied for DTT monitoring both in chemical and biological systems. (en)
  • A new method for specific determination of dithiothreitol (DTT) using micellar electrokinetic chromatography and on-column reaction with reactive disulfide - 2,2-dipyridyldisulfide is described. DTT is in this reaction quantitatively transformed into a mixed disulfide concomitantly with formation of equimolar amount of the 2-thiopyridone that is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm. The concentration of DTT is thus estimated indirectly from the result of 2-thiopyridone determination. The linear detection range for concentration versus peak area for the assay is from 0.05 to 2.5 mM (correlation coefficient 0.993) with a detection limit of 2.5 mM. The inter-day reproducibility of the peak area was 1.35 % and the inter-day reproducibility of the migration time 0.56 %. The method can be applied for DTT monitoring both in chemical and biological systems. (cs)
Title
  • Monitoring of dithiothreitol clearance by means of micellar electrokinetic chromatography
  • Monitoring of dithiothreitol clearance by means of micellar electrokinetic chromatography (en)
  • Monitoring of dithiothreitol clearance by means of micellar electrokinetic chromatography (cs)
skos:prefLabel
  • Monitoring of dithiothreitol clearance by means of micellar electrokinetic chromatography
  • Monitoring of dithiothreitol clearance by means of micellar electrokinetic chromatography (en)
  • Monitoring of dithiothreitol clearance by means of micellar electrokinetic chromatography (cs)
skos:notation
  • RIV/00216224:14310/02:00005543!RIV09-GA0-14310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA525/00/0785)
http://linked.open...iv/cisloPeriodika
  • 1-2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 654181
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/02:00005543
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • DTT; MEKC; on-column (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [3AC567F1AC14]
http://linked.open...i/riv/nazevZdroje
  • Journal of Chromatography B
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 770
http://linked.open...iv/tvurceVysledku
  • Glatz, Zdeněk
  • Olivová, Petra
issn
  • 0378-4347
number of pages
http://localhost/t...ganizacniJednotka
  • 14310
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