About: Determination of glutathione by micellar electrokinetic chromatography and on-column reaction with 2,2-dipyridyldisulphide     Goto   Sponge   NotDistinct   Permalink

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  • A new method for specific determination of glutathione using micellar electrokinetic chromatography and on-column reaction with 2,2 -dipyridyldisulphide is described. 2,2-dipyridyldisulphide and a sample of glutathione are injected consecutively into the capillary as two discrete plugs separated with a short plug of background electrolyte. Due to the differences in the mobilities of the 2,2-dipyridyldisulphide and glutathione, on-column mixing and reaction occur. Glutathione is in this reaction quantitatively transformed into a mixed disulphide concomitantly with formation of equimolar amount of the 2-thiopyridone which is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm. The concentration of glutathione is thus estimated indirectly from the result of 2-thiopyridone determination.
  • A new method for specific determination of glutathione using micellar electrokinetic chromatography and on-column reaction with 2,2 -dipyridyldisulphide is described. 2,2-dipyridyldisulphide and a sample of glutathione are injected consecutively into the capillary as two discrete plugs separated with a short plug of background electrolyte. Due to the differences in the mobilities of the 2,2-dipyridyldisulphide and glutathione, on-column mixing and reaction occur. Glutathione is in this reaction quantitatively transformed into a mixed disulphide concomitantly with formation of equimolar amount of the 2-thiopyridone which is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm. The concentration of glutathione is thus estimated indirectly from the result of 2-thiopyridone determination. (en)
Title
  • Determination of glutathione by micellar electrokinetic chromatography and on-column reaction with 2,2-dipyridyldisulphide
  • Determination of glutathione by micellar electrokinetic chromatography and on-column reaction with 2,2-dipyridyldisulphide (en)
skos:prefLabel
  • Determination of glutathione by micellar electrokinetic chromatography and on-column reaction with 2,2-dipyridyldisulphide
  • Determination of glutathione by micellar electrokinetic chromatography and on-column reaction with 2,2-dipyridyldisulphide (en)
skos:notation
  • RIV/00216224:14310/00:00003265!RIV/2002/GA0/143102/N
http://linked.open.../vavai/riv/strany
  • 155
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA525/00/0785)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
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  • 708441
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  • RIV/00216224:14310/00:00003265
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • glutathione, on-column derivatization, MEKC (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [6E87BADB9BD7]
http://linked.open...v/mistoKonaniAkce
  • Saarbrucken, Germany
http://linked.open...i/riv/mistoVydani
  • Saarbrucken, Germany
http://linked.open...i/riv/nazevZdroje
  • Book of Abstract %2213th International symposium on high performance capillary electrophoresis and related techniques HPCE 2000%22.
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Glatz, Zdeněk
  • Mašlaňová, Hana
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
number of pages
http://purl.org/ne...btex#hasPublisher
  • Universites des Saarlandes
http://localhost/t...ganizacniJednotka
  • 14310
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