About: Comparative Analysis of Salivary Gland Transcriptomes of Phlebotomus orientalis Sand Flies from Endemic and Non-endemic Foci of Visceral Leishmaniasis     Goto   Sponge   NotDistinct   Permalink

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Description
  • Two cDNA libraries were constructed from female salivary glands of P. orientalis from Addis Zemen and Melka Werer. Phylogenetic analysis were performed to evaluate differences between the P. orientalis colonies and to show the relationship with other sand fly species from the subgenus Larroussius. we investigated the humoral antigenicity and cross-reactivity of the salivary proteins and the activity of salivary apyrase and hyaluronidase. Our study expanded the knowledge of salivary gland compounds of sand fly species in the subgenus Larroussius. we showed that P. orientalis is closely related to Phlebotomus tobbi and Phlebotomus perniciosus, whereas Phlebotomus ariasi is evolutionarily more distinct species. We demonstrated that there is no significant difference between the transcriptomes, proteomes or enzymatic properties of the salivary components of Addis Zemen and Melka Werer. Thus, the different epidemiology of VL in these Ethiopian foci cannot be attributed to the salivary gland composition.
  • Two cDNA libraries were constructed from female salivary glands of P. orientalis from Addis Zemen and Melka Werer. Phylogenetic analysis were performed to evaluate differences between the P. orientalis colonies and to show the relationship with other sand fly species from the subgenus Larroussius. we investigated the humoral antigenicity and cross-reactivity of the salivary proteins and the activity of salivary apyrase and hyaluronidase. Our study expanded the knowledge of salivary gland compounds of sand fly species in the subgenus Larroussius. we showed that P. orientalis is closely related to Phlebotomus tobbi and Phlebotomus perniciosus, whereas Phlebotomus ariasi is evolutionarily more distinct species. We demonstrated that there is no significant difference between the transcriptomes, proteomes or enzymatic properties of the salivary components of Addis Zemen and Melka Werer. Thus, the different epidemiology of VL in these Ethiopian foci cannot be attributed to the salivary gland composition. (en)
Title
  • Comparative Analysis of Salivary Gland Transcriptomes of Phlebotomus orientalis Sand Flies from Endemic and Non-endemic Foci of Visceral Leishmaniasis
  • Comparative Analysis of Salivary Gland Transcriptomes of Phlebotomus orientalis Sand Flies from Endemic and Non-endemic Foci of Visceral Leishmaniasis (en)
skos:prefLabel
  • Comparative Analysis of Salivary Gland Transcriptomes of Phlebotomus orientalis Sand Flies from Endemic and Non-endemic Foci of Visceral Leishmaniasis
  • Comparative Analysis of Salivary Gland Transcriptomes of Phlebotomus orientalis Sand Flies from Endemic and Non-endemic Foci of Visceral Leishmaniasis (en)
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  • RIV/00216208:11310/14:10227341!RIV15-MSM-11310___
http://linked.open...avai/riv/aktivita
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  • I, P(GA13-05292S), S
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  • 2
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  • 7928
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  • RIV/00216208:11310/14:10227341
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  • phylogenetic analysis; hyaluronidase activity; braziliensis infection; immune-response; cutaneous leishmaniasis; canine antibody-response; antigenic cross-reactivity; induced platelet-aggregation; delayed-type hypersensitivity; fly lutzomyia-longipalpis (en)
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  • DE - Spolková republika Německo
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  • [CFCDEB1B6D1E]
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  • PLoS Neglected Tropical Diseases [online]
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  • 8
http://linked.open...iv/tvurceVysledku
  • Rohoušová, Iva
  • Vlková, Michaela
  • Volf, Petr
  • Volfová, Věra
  • Barbian, Kent D.
  • Jochim, Ryan C.
  • Valenzuela, Jesus G.
  • Šíma, Michal
  • Gebre-Michael, Teshome
  • Hailu, Asrat
  • Warburg, Alon
  • Jaske, Erin L.
  • Košťálová, Tatiana
  • Ribeiro, Jose M. C.
  • Sumová, Petra
http://linked.open...ain/vavai/riv/wos
  • 000332017500009
issn
  • 1935-2735
number of pages
http://bibframe.org/vocab/doi
  • 10.1371/journal.pntd.0002709
http://localhost/t...ganizacniJednotka
  • 11310
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