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| Description
| - Jaterní progenitorové buňky mohou být zahrnuty v ranných fázích hepatokarcinogeneze indukované chemickými karcinogeny. Použili jsme potkaní jaterní buněčnou linii WB-F344 ke studiu genotoxických efektů vybraných karcinogenních PAHs. S výjimkou dibenzo[a,l]pyrenu a také částečně benzo[g]chrysenu a benz[a]anthracenu, ostatní studované PAHs indukovaly vysoké hladiny mRNA CYP1A1 a CYP1B1. Pozorovány byly rozdílné genotoxické a cytotoxické schopnosti PAHs. Dibenzo[a,l]pyren a v menší míře benzo[a]pyren, benzo[g]chrysen a dibenzo[a,e]pyren vytvářely vysoké hladiny DNA aduktů, doprovázených akumulací fosforylovaného proteinu p53 a indukcí apoptózy. Naproti tomu benz[a]anthracen, chrysen, benzo[b]fluoranthen a dibenzo[a,h]anthracen indukovaly malé množství DNA aduktů a minimální apoptózu. Jaterní progenitorové buňky by mohly být náchylné k tomuto typu genotoxického poškození, což činí buněčnou linii WB-F344 vhodným nástrojem pro studie genotoxických účinků organických kontaminantů v jaterních buňkách. (cs)
- Liver progenitor cells may participate at the early stages of hepatocarcinogenesis induced by chemical carcinogens. We employed rat liver WB-F344 cell line to study genotoxic effects of selected carcinogenic PAHs. With exception of dibenzo[a,l]pyrene, and partly also benzo[g]chrysene and benz[a]anthracene, all other tested PAHs induced high levels of CYP1A1 and CYP1B1 mRNA. We observed distinct genotoxic and cytotoxic potencies of PAHs. Dibenzo[a,l]pyrene, and to a lesser extent also benzo[a]pyrene, benzo[g]chrysene and dibenzo[a,e]pyrene, formed high levels of DNA adducts, accompanied with accumulation of phosphorylated p53 protein and induction of apoptosis. Contrary to that, benz[a]anthracene, chrysene, benzo[b]fluoranthene and dibenzo[a,h]anthracene induced only low amounts of DNA adducts formation and minimal apoptosis. The susceptibility of liver progenitor cells to this type of genotoxic insult makes WB-F344 cell line a useful tool for studies organic contaminants effects in liver cells.
- Liver progenitor cells may participate at the early stages of hepatocarcinogenesis induced by chemical carcinogens. We employed rat liver WB-F344 cell line to study genotoxic effects of selected carcinogenic PAHs. With exception of dibenzo[a,l]pyrene, and partly also benzo[g]chrysene and benz[a]anthracene, all other tested PAHs induced high levels of CYP1A1 and CYP1B1 mRNA. We observed distinct genotoxic and cytotoxic potencies of PAHs. Dibenzo[a,l]pyrene, and to a lesser extent also benzo[a]pyrene, benzo[g]chrysene and dibenzo[a,e]pyrene, formed high levels of DNA adducts, accompanied with accumulation of phosphorylated p53 protein and induction of apoptosis. Contrary to that, benz[a]anthracene, chrysene, benzo[b]fluoranthene and dibenzo[a,h]anthracene induced only low amounts of DNA adducts formation and minimal apoptosis. The susceptibility of liver progenitor cells to this type of genotoxic insult makes WB-F344 cell line a useful tool for studies organic contaminants effects in liver cells. (en)
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| Title
| - DNA adducts formation and induction of apoptosis in rat liver epithelial 'stem-like' cells exposed to carcinogenic polycyclic aromatic hydrocarbons
- Tvorba DNA aduktů a indukce apoptózy v potkaních jaterních epiteliálních 'stem-like' buňkách exponovaných karcinogenním polycyklickým aromatickým uhlovodíkům (cs)
- DNA adducts formation and induction of apoptosis in rat liver epithelial 'stem-like' cells exposed to carcinogenic polycyclic aromatic hydrocarbons (en)
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| skos:prefLabel
| - DNA adducts formation and induction of apoptosis in rat liver epithelial 'stem-like' cells exposed to carcinogenic polycyclic aromatic hydrocarbons
- Tvorba DNA aduktů a indukce apoptózy v potkaních jaterních epiteliálních 'stem-like' buňkách exponovaných karcinogenním polycyklickým aromatickým uhlovodíkům (cs)
- DNA adducts formation and induction of apoptosis in rat liver epithelial 'stem-like' cells exposed to carcinogenic polycyclic aromatic hydrocarbons (en)
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| skos:notation
| - RIV/00027162:_____/08:#0000362!RIV09-MZE-00027162
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GA524/06/0517), Z(AV0Z50040507), Z(AV0Z50040702), Z(AV0Z50390512), Z(MZE0002716201)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00027162:_____/08:#0000362
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - PAHs; genotoxicity; DNA adducts; apoptosis; liver progenitor cells; cytochromes P450; apoptosis (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Nováková, Z.
- Vondráček, Jan
- Machala, Miroslav
- Topinka, J.
- Pěnčíková, Kateřina
- Krčmář, Pavel
- Marvanová, Soňa
- Sevastyanová, O.
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| http://linked.open...ain/vavai/riv/wos
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| http://linked.open...n/vavai/riv/zamer
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