About: Epithelial cells morphology and adhesion on diamonds films deposited and chemically modified by plasma processes     Goto   Sponge   NotDistinct   Permalink

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  • The authors show that nanocrystalline diamond (NCD) thin films prepared by microwave plasma enhanced chemical vapor deposition apparatus with a linear antenna delivery system are well compatible with epithelial cells (5637 human bladder carcinoma) and significantly improve the cell adhesion compared to reference glass substrates. This is attributed to better adhesion of adsorbed layers to diamond as observed by atomic force microscopy (AFM) beneath the cells. Moreover, the cell morphology can be adjusted by appropriate surface treatment of diamond by using hydrogen and oxygen plasma. Cell bodies, cytoplasmic rims, and filopodia were characterized by Peakforce AFM. Oxidized NCD films perform better than other substrates under all conditions (96% of cells adhered well). A thin adsorbed layer formed from culture medium and supplemented with fetal bovine serum (FBS) covered the diamond surface and played an important role in the cell adhesion.
  • The authors show that nanocrystalline diamond (NCD) thin films prepared by microwave plasma enhanced chemical vapor deposition apparatus with a linear antenna delivery system are well compatible with epithelial cells (5637 human bladder carcinoma) and significantly improve the cell adhesion compared to reference glass substrates. This is attributed to better adhesion of adsorbed layers to diamond as observed by atomic force microscopy (AFM) beneath the cells. Moreover, the cell morphology can be adjusted by appropriate surface treatment of diamond by using hydrogen and oxygen plasma. Cell bodies, cytoplasmic rims, and filopodia were characterized by Peakforce AFM. Oxidized NCD films perform better than other substrates under all conditions (96% of cells adhered well). A thin adsorbed layer formed from culture medium and supplemented with fetal bovine serum (FBS) covered the diamond surface and played an important role in the cell adhesion. (en)
Title
  • Epithelial cells morphology and adhesion on diamonds films deposited and chemically modified by plasma processes
  • Epithelial cells morphology and adhesion on diamonds films deposited and chemically modified by plasma processes (en)
skos:prefLabel
  • Epithelial cells morphology and adhesion on diamonds films deposited and chemically modified by plasma processes
  • Epithelial cells morphology and adhesion on diamonds films deposited and chemically modified by plasma processes (en)
skos:notation
  • RIV/68378271:_____/14:00434611!RIV15-GA0-68378271
http://linked.open...avai/riv/aktivita
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  • I, P(GAP108/12/0996), P(KAN400100701)
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  • 3
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  • 14871
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  • RIV/68378271:_____/14:00434611
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  • nanocrystalline diamond (NCD); AFM; surfaces; DNA (en)
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  • DE - Spolková republika Německo
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  • [0B775D2612AF]
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  • Biointerphases
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  • 9
http://linked.open...iv/tvurceVysledku
  • Krátká, Marie
  • Rezek, Bohuslav
  • Ukraintsev, Egor
  • Fendrych, František
  • Taylor, Andrew
  • Mandys, V.
http://linked.open...ain/vavai/riv/wos
  • 000340893400013
issn
  • 1934-8630
number of pages
http://bibframe.org/vocab/doi
  • 10.1116/1.4890471
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