About: Detection of allergenic parvalbumin of Atlantic and Pacific herrings in fish products by PCR

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About: Detection of allergenic parvalbumin of Atlantic and Pacific herrings in fish products by PCR Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The conventional polymerase chain reaction (PCR) method to detect the major allergenic protein parvalbumin beta 2 of Atlantic herring (Clupea harengus) and Pacific herring (Clupea pallasii) was developed. The specific set of primers for the amplification of the partial genomic sequence of the pvalb 2 gene encoding the main fish allergen of both herrings was designed and applied to the investigation of 24 commercial fish products. The targeted amplicon size was 189bp of pvalb 2 gene of Atlantic herring and Pacific herring. As the internal amplification control, the DNA of 18S rRNA gene for eukaryotes (141bp) was successfully used. The specificity of designed primer pair using 26 various fish species was assessed. The intrinsic detection limit was 10pg mu l(-1) of the present specific DNA. Atlantic herring or Pacific herring allergenic parvalbumins were detected in 22 investigated fish products in conformity with the package declaration. Two fish products were negative in spite of the declaration. The proposed PCR method is specific enough and can be used for the detection of Atlantic and Pacific herrings' major allergen parvalbumin beta 2 in fish food products. The conventional polymerase chain reaction (PCR) method to detect the major allergenic protein parvalbumin beta 2 of Atlantic herring (Clupea harengus) and Pacific herring (Clupea pallasii) was developed. The specific set of primers for the amplification of the partial genomic sequence of the pvalb 2 gene encoding the main fish allergen of both herrings was designed and applied to the investigation of 24 commercial fish products. The targeted amplicon size was 189bp of pvalb 2 gene of Atlantic herring and Pacific herring. As the internal amplification control, the DNA of 18S rRNA gene for eukaryotes (141bp) was successfully used. The specificity of designed primer pair using 26 various fish species was assessed. The intrinsic detection limit was 10pg mu l(-1) of the present specific DNA. Atlantic herring or Pacific herring allergenic parvalbumins were detected in 22 investigated fish products in conformity with the package declaration. Two fish products were negative in spite of the declaration. The proposed PCR method is specific enough and can be used for the detection of Atlantic and Pacific herrings' major allergen parvalbumin beta 2 in fish food products. (en)
Title	Detection of allergenic parvalbumin of Atlantic and Pacific herrings in fish products by PCR Detection of allergenic parvalbumin of Atlantic and Pacific herrings in fish products by PCR (en)
skos:prefLabel	Detection of allergenic parvalbumin of Atlantic and Pacific herrings in fish products by PCR Detection of allergenic parvalbumin of Atlantic and Pacific herrings in fish products by PCR (en)
skos:notation	RIV/62157124:16270/13:43873338!RIV15-MSM-16270___
http://linked.open...avai/riv/aktivita	I Z
http://linked.open...avai/riv/aktivity	I, Z(MZE0002716202)
http://linked.open...iv/cisloPeriodika	10
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Tremlová, Bohuslava
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Veterinární a farmaceutická univerzita Brno / Fakulta veterinární hygieny a ekologie
http://linked.open...dnocenehoVysledku	68721
http://linked.open...ai/riv/idVysledku	RIV/62157124:16270/13:43873338
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	PCR; canned products; food allergens; DNA; fish allergens (en)
http://linked.open.../riv/klicoveSlovo	canned products fish allergens food allergens DNA PCR
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[A366770DA852]
http://linked.open...i/riv/nazevZdroje	Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment
http://linked.open...in/vavai/riv/obor	GM
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	30
http://linked.open...iv/tvurceVysledku	Renčová, Eva Tremlová, Bohuslava Kostelníková, Darina
http://linked.open...ain/vavai/riv/wos	000324903700001
http://linked.open...n/vavai/riv/zamer	Animal disease research, prevention and food chain protection
issn	1944-0049
number of pages	5 (xsd:int)
http://bibframe.org/vocab/doi	10.1080/19440049.2013.817024
http://localhost/t...ganizacniJednotka	16270

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