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| Description
| - Heavy metals are classified as priority pollutants monitored in environments. Heavy metals are not biodegradable, having ability to accumulate in organisms. Cadmium is one of the most toxic heavy metal ions in the environment due to its high mobility and severe toxicity to the organisms. Zinc is an essential micronutrient for plants but can be highly toxic when present at excessive concentration. We aimed at investigation of detoxification mechanisms of maize plants (Zea mays L.) treated with the following combinations of zinc(II) and cadmium(II) ions 0 mM Zn2+ + 0 mM Cd2+; 100 mM Zn2+ + 0 mM Cd2+; 0 mM Zn2+ + 100 mM Cd2+; 10 mM Zn2+ + 100 mM Cd2+; 50 mM Zn2+ + 100 mM Cd2+; 75 mM Zn2+ + 100 mM Cd2+ and 100 mM Zn2+ + 100 mM Cd2+ for 10 days. In addition to growth parameters, we electrochemically determined the metal content in plants. To determine the antioxidant activity, we chose three methods FRAP, DPPH and ABTS, which differ in the spectrum of molecules with possible antioxidant activity. Pearson's correlation coefficient was found by comparison of FRAP and ABTS methods - rP = 0.7967 (Bradford) and 0.7957 (Pyrogallol Red). Pearson's correlation coefficients indicates mutual correlation of both DPPH and ABTS methods - rP = 0.8292 (Bradford) and 0.8737 (Pyrogallol Red). The highest correlation of Pearson's coefficients was found in comparison between DPPH and FRAP methods (0.9407 -- Bradford and 0.9211 Pyrogallol Red), suggesting a very high dependence of the two methods. Thus, our attention was focused on the determination of activities of alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP). We were also focused on the monitoring of the GST activity. In addition, we monitored its correlation with total content of thiol compounds (-SH) measured spectrometrically using Ellman's method. Content of thiol compounds, such as reduced (GSH) and oxidized (GSSG) glutathione, and phytochelatins (PC2, PC5) was determined by HPLC--ED. The hi
- Heavy metals are classified as priority pollutants monitored in environments. Heavy metals are not biodegradable, having ability to accumulate in organisms. Cadmium is one of the most toxic heavy metal ions in the environment due to its high mobility and severe toxicity to the organisms. Zinc is an essential micronutrient for plants but can be highly toxic when present at excessive concentration. We aimed at investigation of detoxification mechanisms of maize plants (Zea mays L.) treated with the following combinations of zinc(II) and cadmium(II) ions 0 mM Zn2+ + 0 mM Cd2+; 100 mM Zn2+ + 0 mM Cd2+; 0 mM Zn2+ + 100 mM Cd2+; 10 mM Zn2+ + 100 mM Cd2+; 50 mM Zn2+ + 100 mM Cd2+; 75 mM Zn2+ + 100 mM Cd2+ and 100 mM Zn2+ + 100 mM Cd2+ for 10 days. In addition to growth parameters, we electrochemically determined the metal content in plants. To determine the antioxidant activity, we chose three methods FRAP, DPPH and ABTS, which differ in the spectrum of molecules with possible antioxidant activity. Pearson's correlation coefficient was found by comparison of FRAP and ABTS methods - rP = 0.7967 (Bradford) and 0.7957 (Pyrogallol Red). Pearson's correlation coefficients indicates mutual correlation of both DPPH and ABTS methods - rP = 0.8292 (Bradford) and 0.8737 (Pyrogallol Red). The highest correlation of Pearson's coefficients was found in comparison between DPPH and FRAP methods (0.9407 -- Bradford and 0.9211 Pyrogallol Red), suggesting a very high dependence of the two methods. Thus, our attention was focused on the determination of activities of alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP). We were also focused on the monitoring of the GST activity. In addition, we monitored its correlation with total content of thiol compounds (-SH) measured spectrometrically using Ellman's method. Content of thiol compounds, such as reduced (GSH) and oxidized (GSSG) glutathione, and phytochelatins (PC2, PC5) was determined by HPLC--ED. The hi (en)
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| Title
| - Study of cadmium and zinc interaction in maize
- Study of cadmium and zinc interaction in maize (en)
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| skos:prefLabel
| - Study of cadmium and zinc interaction in maize
- Study of cadmium and zinc interaction in maize (en)
|
| skos:notation
| - RIV/62156489:43210/11:00180359!RIV12-MSM-43210___
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| http://linked.open...avai/predkladatel
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/62156489:43210/11:00180359
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - heavy metals; interaction; trace elements (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...v/mistoKonaniAkce
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| http://linked.open...i/riv/mistoVydani
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...iv/tvurceVysledku
| - Adam, Vojtěch
- Babula, Petr
- Dočekalová, Hana
- Kizek, René
- Kleckerová, Andrea
- Kryštofová, Olga
- Sochor, Jiří
- Zítka, Ondřej
- Šobrová, Pavlína
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| http://linked.open...vavai/riv/typAkce
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| http://linked.open.../riv/zahajeniAkce
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| number of pages
| |
| http://purl.org/ne...btex#hasPublisher
| - Mendelova zemědělská a lesnická univerzita v Brně
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| https://schema.org/isbn
| |
| http://localhost/t...ganizacniJednotka
| |
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