About: A novel method for analysis of plants' resistance to heavy metals

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	https://mnet.mendelu.cz/mendelnet2011/mendelnet_2011_fulltext.pdf
Description	Nowadays many less developed countries has many problems concerning about pollution in environment caused by heavy metals as cadmium, lead or mercury. The main aim of this work was to optimize method for determination of activity of main plant protective mechanism against the heavy metals. Sulphur rich peptides phytochlatins (PC's) are physiologically active compounds because they are able of immobilization of the toxic heavy metal directly in the plant. Most occurring of PC's is phytochelatin2 (PC-2) which is synthesized by phytochelatin synthase (PCS). We focused on developing of the method based on high performance liquid chromatography coupled with electrochemical detector (HPLC-ED) for determination of PC-2 because it might serve as suitable tool for determination of phytochelatin synthase activity. PCS is the best activated by cadmium ions. We used a model with BY-2 tobacco cells. We conducted the in vivo 3 day cultivation experiment where BY-2 cells were treated by various concentrations of cadmium. We then homogenized the cells and immediately analyzed the extracts by optimized HPLC-ED method. Moreover we observed that with higher concentrations of applied cadmium there was increasing of amount of PC-2. Nowadays many less developed countries has many problems concerning about pollution in environment caused by heavy metals as cadmium, lead or mercury. The main aim of this work was to optimize method for determination of activity of main plant protective mechanism against the heavy metals. Sulphur rich peptides phytochlatins (PC's) are physiologically active compounds because they are able of immobilization of the toxic heavy metal directly in the plant. Most occurring of PC's is phytochelatin2 (PC-2) which is synthesized by phytochelatin synthase (PCS). We focused on developing of the method based on high performance liquid chromatography coupled with electrochemical detector (HPLC-ED) for determination of PC-2 because it might serve as suitable tool for determination of phytochelatin synthase activity. PCS is the best activated by cadmium ions. We used a model with BY-2 tobacco cells. We conducted the in vivo 3 day cultivation experiment where BY-2 cells were treated by various concentrations of cadmium. We then homogenized the cells and immediately analyzed the extracts by optimized HPLC-ED method. Moreover we observed that with higher concentrations of applied cadmium there was increasing of amount of PC-2. (en)
Title	A novel method for analysis of plants' resistance to heavy metals A novel method for analysis of plants' resistance to heavy metals (en)
skos:prefLabel	A novel method for analysis of plants' resistance to heavy metals A novel method for analysis of plants' resistance to heavy metals (en)
skos:notation	RIV/62156489:43210/11:00180218!RIV15-MSM-43210___
http://linked.open...avai/riv/aktivita	S
http://linked.open...avai/riv/aktivity	S
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Kryštofová, Olga Adam, Vojtěch Zehnálek, Josef Beklová, Miroslava Kizek, René Šobrová, Pavlína Zítka, Ondřej
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mendelova univerzita v Brně / Agronomická fakulta
http://linked.open...dnocenehoVysledku	184021
http://linked.open...ai/riv/idVysledku	RIV/62156489:43210/11:00180218
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	phytochelatin synthese; coulometric detection; heavy metals (en)
http://linked.open.../riv/klicoveSlovo	heavy metals phytochelatin synthese coulometric detection
http://linked.open...ontrolniKodProRIV	[C1C6F64FBB0D]
http://linked.open...v/mistoKonaniAkce	Brno
http://linked.open...i/riv/mistoVydani	Brno
http://linked.open...i/riv/nazevZdroje	MendelNet 2011
http://linked.open...in/vavai/riv/obor	GE
http://linked.open...ichTvurcuVysledku	7 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...UplatneniVysledku	2011
http://linked.open...iv/tvurceVysledku	Adam, Vojtěch Beklová, Miroslava Kizek, René Kryštofová, Olga Zehnálek, Josef Zítka, Ondřej Šobrová, Pavlína
http://linked.open...vavai/riv/typAkce	CST - Celostátní
http://linked.open...ain/vavai/riv/wos	341783400130
http://linked.open.../riv/zahajeniAkce	2011-01-01 (xsd:date)
number of pages	5 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Mendelova zemědělská a lesnická univerzita v Brně
https://schema.org/isbn	978-80-7375-563-8
http://localhost/t...ganizacniJednotka	43210

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