About: Employment of HPLC with coulometric detection for analysis of phytochelatin synthase activity

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Phytochelatins are physiologically active peptides which have to be synthesized by phytochelatin synthase (PCS). The main aim of this study was to optimize high performance liquid chromatography coupled with electrochemical detector (HPLC-ED) as suitable tool for determination of the phytochelatin synthase activity. Coulometric detector with porous graphite working electrode was used. Firstly we optimized a separation of two compounds which are important for monitoring of PCS activity. It was glutathione reduced as substrate for the reaction and fytochelatin-2 as a product. PCS is the best activated by cadmium ions. We used a model with BY-2 tobacco cells. We conducted the in vivo 3 day cultivation experiment where BY-2 cells were treated by various concentrations of cadmium. We then homogenized the cells and immediately analyzed the extracts by optimized HPLC-ED method. Moreover we observed that with higher concentrations of applied cadmium there was increasing of amount of PC-2. The lowest concentration of the toxic metal ions caused almost three times enhancing PCS activity compared to control samples. Phytochelatins are physiologically active peptides which have to be synthesized by phytochelatin synthase (PCS). The main aim of this study was to optimize high performance liquid chromatography coupled with electrochemical detector (HPLC-ED) as suitable tool for determination of the phytochelatin synthase activity. Coulometric detector with porous graphite working electrode was used. Firstly we optimized a separation of two compounds which are important for monitoring of PCS activity. It was glutathione reduced as substrate for the reaction and fytochelatin-2 as a product. PCS is the best activated by cadmium ions. We used a model with BY-2 tobacco cells. We conducted the in vivo 3 day cultivation experiment where BY-2 cells were treated by various concentrations of cadmium. We then homogenized the cells and immediately analyzed the extracts by optimized HPLC-ED method. Moreover we observed that with higher concentrations of applied cadmium there was increasing of amount of PC-2. The lowest concentration of the toxic metal ions caused almost three times enhancing PCS activity compared to control samples. (en)
Title	Employment of HPLC with coulometric detection for analysis of phytochelatin synthase activity Employment of HPLC with coulometric detection for analysis of phytochelatin synthase activity (en)
skos:prefLabel	Employment of HPLC with coulometric detection for analysis of phytochelatin synthase activity Employment of HPLC with coulometric detection for analysis of phytochelatin synthase activity (en)
skos:notation	RIV/62156489:43210/11:00174192!RIV12-GA0-43210___
http://linked.open...avai/predkladatel	Agronomická fakulta
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA522/07/0692), P(GD204/09/H002)
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Kryštofová, Olga Adam, Vojtěch Zehnálek, Josef Beklová, Miroslava Kizek, René Šobrová, Pavlína Zítka, Ondřej
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mendelova univerzita v Brně / Agronomická fakulta
http://linked.open...dnocenehoVysledku	197422
http://linked.open...ai/riv/idVysledku	RIV/62156489:43210/11:00174192
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	phytochelatin synthase; coulometric analysis; HPLC (en)
http://linked.open.../riv/klicoveSlovo	coulometric analysis phytochelatin synthase HPLC
http://linked.open...ontrolniKodProRIV	[C7DD46FF9C9A]
http://linked.open...v/mistoKonaniAkce	MENDELU
http://linked.open...i/riv/mistoVydani	Brno
http://linked.open...i/riv/nazevZdroje	XI. pracovní setkání fyzikálních chemiků a elektrochemiků
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	7 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Plant Developmental Biology and Genetics Study of thiol compounds production at plants - use in phytoremediation technologies
http://linked.open...UplatneniVysledku	2011
http://linked.open...iv/tvurceVysledku	Adam, Vojtěch Beklová, Miroslava Kizek, René Kryštofová, Olga Zehnálek, Josef Zítka, Ondřej Šobrová, Pavlína
http://linked.open...vavai/riv/typAkce	CST - Celostátní
http://linked.open.../riv/zahajeniAkce	2011-01-01 (xsd:date)
number of pages	4 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Mendelova zemědělská a lesnická univerzita v Brně
https://schema.org/isbn	978-80-7375-514-0
http://localhost/t...ganizacniJednotka	43210

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