About: Electrochemical oxidation of proteins using ionic liquids as solubilizers, adsorption solvents and electrolytes

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1016/j.electacta.2013.06.115
Description	This study focuses on application of room temperature ionic liquids (RTILs) as solubilizers, adsorption solvents and supporting electrolytes for electrochemical analysis of human and bovine serum albumins, HSA and BSA. The proteins were analyzed by ex situ, adsorptive transfer, square-wave voltammetry (SWV) at a basal-plane pyrolytic graphite electrode after solubilization using imidazolium- and ammonium-based RTILs. The application of RTILs enabled SWV scan from 0 to +1.5 V (vs. Ag/AgCl/3 M KCl) without interference with the anodic response of the proteins. Concretely, Tyr (Y) and Trp (W) oxidation currentsof HSA and BSA, peak Y&W around +0.85 V, were observed and characterized under different RTILs and RTIL/water conditions. The electrochemical data were supported by electrophoresis under denaturing and native conditions. These provided evidence for the structural changes and stability of the studied proteinsin the presence of RTILs. The data acquired using BSA and HSA model proteins, could be used in further applications of RTILs in protein electrochemistry and for developing new protein sensing strategies. This study focuses on application of room temperature ionic liquids (RTILs) as solubilizers, adsorption solvents and supporting electrolytes for electrochemical analysis of human and bovine serum albumins, HSA and BSA. The proteins were analyzed by ex situ, adsorptive transfer, square-wave voltammetry (SWV) at a basal-plane pyrolytic graphite electrode after solubilization using imidazolium- and ammonium-based RTILs. The application of RTILs enabled SWV scan from 0 to +1.5 V (vs. Ag/AgCl/3 M KCl) without interference with the anodic response of the proteins. Concretely, Tyr (Y) and Trp (W) oxidation currentsof HSA and BSA, peak Y&W around +0.85 V, were observed and characterized under different RTILs and RTIL/water conditions. The electrochemical data were supported by electrophoresis under denaturing and native conditions. These provided evidence for the structural changes and stability of the studied proteinsin the presence of RTILs. The data acquired using BSA and HSA model proteins, could be used in further applications of RTILs in protein electrochemistry and for developing new protein sensing strategies. (en)
Title	Electrochemical oxidation of proteins using ionic liquids as solubilizers, adsorption solvents and electrolytes Electrochemical oxidation of proteins using ionic liquids as solubilizers, adsorption solvents and electrolytes (en)
skos:prefLabel	Electrochemical oxidation of proteins using ionic liquids as solubilizers, adsorption solvents and electrolytes Electrochemical oxidation of proteins using ionic liquids as solubilizers, adsorption solvents and electrolytes (en)
skos:notation	RIV/61989592:15110/14:33148910!RIV15-MSM-15110___
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(EE2.3.20.0057)
http://linked.open...iv/cisloPeriodika	April
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Vacek, Jan Vrba, Jiří Zatloukalová, Martina
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Lékařská fakulta
http://linked.open...dnocenehoVysledku	14114
http://linked.open...ai/riv/idVysledku	RIV/61989592:15110/14:33148910
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Electrolytes; Protein stability; Electrochemical sensing; Ionic liquid; Protein (en)
http://linked.open.../riv/klicoveSlovo	Electrochemical sensing Protein stability Protein Electrolytes Ionic liquid
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[FAEC2C160ED3]
http://linked.open...i/riv/nazevZdroje	Electrochimica Acta
http://linked.open...in/vavai/riv/obor	CG
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Progress and internationalization of biophysical research at Faculty of Science, Palacky University in Olomouc
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	126
http://linked.open...iv/tvurceVysledku	Vacek, Jan Kubala, Martin Vrba, Jiří Zatloukalová, Martina
issn	0013-4686
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.electacta.2013.06.115
http://localhost/t...ganizacniJednotka	15110

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