About: Effect of hydrophobic interactions on properties and stability of DNA-polyelectrolyte complexes

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About: Effect of hydrophobic interactions on properties and stability of DNA-polyelectrolyte complexes Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Polyplexes are polyelectrolyte complexes of DNA and polycations, designed for potential gene delivery. We investigated the properties of new polyplexes formed from cholesterol-modified polycations and DNA. Three complexes were tested; their cholesterol contents were 1.4, 6.3, and 8.7 mol %. UV spectroscopy and fluorescence assay using ethidium bromide proved the formation of polyplexes. The kinetics of turbidity of polyplexes solutions in physiological solution showed that the colloid stability of polyplexes increases with increasing content of cholesterol in polycations. DLS, SLS, electrophoretic light scattering, SAXS, and atomic force microscopy were used for characterization of polyplexes. The observed hydrodynamic radii of polyplexes were in the range of 30-60 nm. The properties of polyplex particles depend, in addition to polycation structure, on the rate of polycation addition to DNA solutions. Polyplexes are polyelectrolyte complexes of DNA and polycations, designed for potential gene delivery. We investigated the properties of new polyplexes formed from cholesterol-modified polycations and DNA. Three complexes were tested; their cholesterol contents were 1.4, 6.3, and 8.7 mol %. UV spectroscopy and fluorescence assay using ethidium bromide proved the formation of polyplexes. The kinetics of turbidity of polyplexes solutions in physiological solution showed that the colloid stability of polyplexes increases with increasing content of cholesterol in polycations. DLS, SLS, electrophoretic light scattering, SAXS, and atomic force microscopy were used for characterization of polyplexes. The observed hydrodynamic radii of polyplexes were in the range of 30-60 nm. The properties of polyplex particles depend, in addition to polycation structure, on the rate of polycation addition to DNA solutions. (en)
Title	Effect of hydrophobic interactions on properties and stability of DNA-polyelectrolyte complexes Effect of hydrophobic interactions on properties and stability of DNA-polyelectrolyte complexes (en)
skos:prefLabel	Effect of hydrophobic interactions on properties and stability of DNA-polyelectrolyte complexes Effect of hydrophobic interactions on properties and stability of DNA-polyelectrolyte complexes (en)
skos:notation	RIV/61389013:_____/10:00341720!RIV11-GA0-61389013
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA202/09/2078), Z(AV0Z40500505)
http://linked.open...iv/cisloPeriodika	7
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Štěpánek, Petr Špírková, Milena Starovoytova, Larisa Koňák, Čestmír Filippov, Sergey
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav makromolekulární chemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	256018
http://linked.open...ai/riv/idVysledku	RIV/61389013:_____/10:00341720
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DNA; polyplex; cholesterol (en)
http://linked.open.../riv/klicoveSlovo	polyplex DNA cholesterol
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[03F4DF85BAF3]
http://linked.open...i/riv/nazevZdroje	Langmuir
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Bioinspired nanoparticles sensitive to environment stimuli
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	26
http://linked.open...iv/tvurceVysledku	Filippov, Sergey Starovoytova, Larisa Štěpánek, Petr Špírková, Milena Koňák, Čestmír Kopečková, P.
http://linked.open...ain/vavai/riv/wos	000275995100068
http://linked.open...n/vavai/riv/zamer	Progresivní makromolekulární materiály a supramolekulární systémy: syntéza a studium vlastností, jevů a možností využití pro speciální aplikace a moderní technologie
issn	0743-7463
number of pages	8 (xsd:int)

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