About: Determination of Urinary 8-hydroxy-2''-deoxyguanosine in Obese Patiens by HPLC with Electrochemical Detection

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Pro analýzu 8-hydroxy-2´-deoxyguanosinu (8-OHdG) v moči obézních pacientů metodou HPLC byl testován jednoduchý amperometrický detektor. Analyt byl extrahován z moči s použitím jednostupňové extrakce na pevné fázi s průměrným výtěžkem 71%. HPLC byla prováděna na C-18 analytických kolonách za izokratických podmínek. Zjištěný limit detekce 8-OHdG (3nM) byl srovnatelný s limitem dosaženým při použití komerčně dostupného amperometrického detektoru. Postup měření byl pak použit pro určení hladin 8-OHdG v moči 10 obézních pacientů s výsledkem 32+/-27,1 nM, který se příliš nelišil od hodnoty zjištěné u zdravých jedinců. (cs) A simple amperometric detector with a platinum tubular electrode was tested for the determination of urinary 8-hydroxy-2''-deoxyguanosine (8-OHdG) by high-performance liquid chromatography. Analyte was extracted from human urine using a single-step solid-phase extraction with the average recovery of 71 +/- 7%. Analysis was carried out on a C-18 analytical column under isocratic conditions. The limit of detection of 8-OHdG of 3 nM (S/N = 3) was comparable with that achieved using the conventional amperometric detector. Procedure was used for the determination of 8-OHdG levels in 10 obese patients (body-mass index > 30 kg m(-2)) yielding the mean concentration 32 +/- 27.1 nM of 8-OHdG or 1.85 +/- 0.72 .mu.mol 8-OHdG mol(-1) creatinine (4.63 +/- 1.8. mu.g 8-OHdG g(-1) creatinine), which is not much different from the levels found in healthy individuals. A simple amperometric detector with a platinum tubular electrode was tested for the determination of urinary 8-hydroxy-2''-deoxyguanosine (8-OHdG) by high-performance liquid chromatography. Analyte was extracted from human urine using a single-step solid-phase extraction with the average recovery of 71 +/- 7%. Analysis was carried out on a C-18 analytical column under isocratic conditions. The limit of detection of 8-OHdG of 3 nM (S/N = 3) was comparable with that achieved using the conventional amperometric detector. Procedure was used for the determination of 8-OHdG levels in 10 obese patients (body-mass index > 30 kg m(-2)) yielding the mean concentration 32 +/- 27.1 nM of 8-OHdG or 1.85 +/- 0.72 .mu.mol 8-OHdG mol(-1) creatinine (4.63 +/- 1.8. mu.g 8-OHdG g(-1) creatinine), which is not much different from the levels found in healthy individuals. (en)
Title	Determination of Urinary 8-hydroxy-2''-deoxyguanosine in Obese Patiens by HPLC with Electrochemical Detection Determination of Urinary 8-hydroxy-2''-deoxyguanosine in Obese Patiens by HPLC with Electrochemical Detection (en) Analýza 8-hydroxy-2´-deoxyguanosinu v moči obézních pacientů metodou HPLC s elektrochemickou detekcí (cs)
skos:prefLabel	Determination of Urinary 8-hydroxy-2''-deoxyguanosine in Obese Patiens by HPLC with Electrochemical Detection Determination of Urinary 8-hydroxy-2''-deoxyguanosine in Obese Patiens by HPLC with Electrochemical Detection (en) Analýza 8-hydroxy-2´-deoxyguanosinu v moči obézních pacientů metodou HPLC s elektrochemickou detekcí (cs)
skos:notation	RIV/61388955:_____/04:00101099!RIV/2005/AV0/A54005/N
http://linked.open.../vavai/riv/strany	107;110
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/01/0653), Z(AV0Z4040901)
http://linked.open...iv/cisloPeriodika	-
http://linked.open...vai/riv/dodaniDat	2005
http://linked.open...aciTvurceVysledku	Langmaier, Jan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav fyzikální chemie J. Heyrovského AV ČR, v.v.i.
http://linked.open...dnocenehoVysledku	560141
http://linked.open...ai/riv/idVysledku	RIV/61388955:_____/04:00101099
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	8-hydroxy-2''''-deoxyguanosine; DNA; obese patient (en)
http://linked.open.../riv/klicoveSlovo	8-hydroxy-2''''-deoxyguanosine DNA obese patient
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[FC0E0D1541D4]
http://linked.open...i/riv/nazevZdroje	Analytica Chimica Acta
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	New electrochemical methods for quantitative detection of 8-hydroxy-2´-deoxyguanosine in urine
http://linked.open...UplatneniVysledku	2004
http://linked.open...v/svazekPeriodika	516
http://linked.open...iv/tvurceVysledku	Langmaier, Jan Opekar, F. Samcová, E. Marhol, P.
http://linked.open...n/vavai/riv/zamer	Objasňování zákonitostí vztahů struktury a vlastností - zejména dynamiky a chemické reaktivity - látek a molekulárních systémů
issn	0003-2670
number of pages	4 (xsd:int)

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