About: DNA interaction with zinc(II) ions

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We focused on interactions of Zn(II) with DNA in this study. These interactions were monitored usingUV/vis spectrophotometry and gel electrophoresis. Firstly, we isolated and amplified 498 bp fragmentof DNA. Samples were obtained by incubation of DNA fragment with Zn(II) for 60 min at 25 C. After incubation, the samples were dialyzed and analyzed immediately. In this way, DNA was converted intoa metal bound DNA (Zn–DNA). Interaction of Zn(II) with DNA caused change in the absorption spectrum(190–350 nm) and decrease in the melting temperature (Tm) of Zn–DNA. Spectrophotometric (UV/vis)analysis showed that increasing concentrations of zinc(II) ions led to the increase in the absorbance at200 nm and decrease in absorbance at 251 nm. We focused on interactions of Zn(II) with DNA in this study. These interactions were monitored usingUV/vis spectrophotometry and gel electrophoresis. Firstly, we isolated and amplified 498 bp fragmentof DNA. Samples were obtained by incubation of DNA fragment with Zn(II) for 60 min at 25 C. After incubation, the samples were dialyzed and analyzed immediately. In this way, DNA was converted intoa metal bound DNA (Zn–DNA). Interaction of Zn(II) with DNA caused change in the absorption spectrum(190–350 nm) and decrease in the melting temperature (Tm) of Zn–DNA. Spectrophotometric (UV/vis)analysis showed that increasing concentrations of zinc(II) ions led to the increase in the absorbance at200 nm and decrease in absorbance at 251 nm. (en)
Title	DNA interaction with zinc(II) ions DNA interaction with zinc(II) ions (en)
skos:prefLabel	DNA interaction with zinc(II) ions DNA interaction with zinc(II) ions (en)
skos:notation	RIV/00216305:26620/14:PU108609!RIV15-MSM-26620___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(EE2.4.31.0023), P(GAP102/11/1068)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Ryvolová, Markéta Křížková, Soňa Babula, Petr Trnková, Libuše Adam, Vojtěch Zehnálek, Josef Kizek, René Ruttkay-Nedecký, Branislav Dostálová, Simona Kopel, Pavel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoké učení technické v Brně / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	11997
http://linked.open...ai/riv/idVysledku	RIV/00216305:26620/14:PU108609
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	UV/vis spectrophotometry, Gel electrophoresis, Melting temperature (en)
http://linked.open.../riv/klicoveSlovo	Melting temperature UV/vis spectrophotometry Gel electrophoresis
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[7CC974A2942C]
http://linked.open...i/riv/nazevZdroje	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
http://linked.open...in/vavai/riv/obor	CG
http://linked.open...ichTvurcuVysledku	10 (xsd:int)
http://linked.open...cetTvurcuVysledku	13 (xsd:int)
http://linked.open...vavai/riv/projekt	Partner Network for Bionanotechnological and Metallomic Research Nano-Electro-Bio-Tools for Biochemical and Molecularly-Biological Studies of Eukaryotic Cells (NanoBioTECell)
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	64
http://linked.open...iv/tvurceVysledku	Adam, Vojtěch Babula, Petr Kizek, René Křížková, Soňa Trnková, Libuše Vaculovičová, Markéta Zehnálek, Josef Dostálová, Simona Ruttkay-Nedecký, Branislav Šmerková, Kristýna Kopel, Pavel Nejdl, Lukáš Kudr, Jiří
issn	0141-8130
number of pages	7 (xsd:int)
http://localhost/t...ganizacniJednotka	26620

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