About: Miniaturized electrochemical detector as a tool for detection of DNA amplified by PCR

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	This paper reports on the analysis of specific sequence of Phage Lambda DNA amplified by PCR. Agarose gel electrophoresis, gel electrophoresis on chip and stationary electrochemical instrument were employed for detection of amplicons obtained after 2, 4, 6, 8, 10, 15, 20, 25, 30 and 35 cycles. In the case of agarose gel electrophoresis the lowest detectable amount of DNA was obtained after 15 PCR cycles. Gel electrophoresis on chip offers higher sensitivity because the lowest detectable amount of amplicons by this technique was obtained after eight PCR cycles. Further we employed square wave voltammetry and various working electrodes (hanging mercury drop electrode, screen-printed carbon electrode and carbon-nanotube-based screen-printed electrodes) to detect amplicons. Amplicons obtained even after two cycles were detectable at all electrodes. To improve the selectivity of electrochemical detection carbon nanoelectrodes were off-line coupled with gel electrophoresis. Into the agarose gel the electrod This paper reports on the analysis of specific sequence of Phage Lambda DNA amplified by PCR. Agarose gel electrophoresis, gel electrophoresis on chip and stationary electrochemical instrument were employed for detection of amplicons obtained after 2, 4, 6, 8, 10, 15, 20, 25, 30 and 35 cycles. In the case of agarose gel electrophoresis the lowest detectable amount of DNA was obtained after 15 PCR cycles. Gel electrophoresis on chip offers higher sensitivity because the lowest detectable amount of amplicons by this technique was obtained after eight PCR cycles. Further we employed square wave voltammetry and various working electrodes (hanging mercury drop electrode, screen-printed carbon electrode and carbon-nanotube-based screen-printed electrodes) to detect amplicons. Amplicons obtained even after two cycles were detectable at all electrodes. To improve the selectivity of electrochemical detection carbon nanoelectrodes were off-line coupled with gel electrophoresis. Into the agarose gel the electrod (en)
Title	Miniaturized electrochemical detector as a tool for detection of DNA amplified by PCR Miniaturized electrochemical detector as a tool for detection of DNA amplified by PCR (en)
skos:prefLabel	Miniaturized electrochemical detector as a tool for detection of DNA amplified by PCR Miniaturized electrochemical detector as a tool for detection of DNA amplified by PCR (en)
skos:notation	RIV/00216305:26220/09:PU85269!RIV10-AV0-26220___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(KAN208130801)
http://linked.open...iv/cisloPeriodika	24
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Hubálek, Jaromír
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoké učení technické v Brně / Fakulta elektrotechniky a komunikačních technologií
http://linked.open...dnocenehoVysledku	326413
http://linked.open...ai/riv/idVysledku	RIV/00216305:26220/09:PU85269
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Carbon nanotubes, DNA, Gel electrophoresis on chip, PCR, Voltammetry (en)
http://linked.open.../riv/klicoveSlovo	DNA PCR Gel electrophoresis on chip Carbon nanotubes Voltammetry
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[5E99A23BB1EB]
http://linked.open...i/riv/nazevZdroje	Electrophoresis
http://linked.open...in/vavai/riv/obor	CG
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	New design and exploiting nanobiosensors and nanosensors to target medicine (NANOSEMED)
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	2008 (29)
http://linked.open...iv/tvurceVysledku	Adam, Vojtěch Hubálek, Jaromír Húska, Dalibor Kizek, René
issn	0173-0835
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	26220

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