About: Piezoelectric biosensors for detection of microorganisms

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://web2.mendelu.cz/af_239_nanotech/data/soubory/sbornik2014.pdf#page=34
Description	Piezoelectric biosensors provide fast, specific and economic way of detection for a wide range of analytes ranging from metabolites to microorganisms. Specific antibodies were immobilised on gold electrodes of piezoelectric crystal and the biosensor was applied for real-time detection of model microorganisms. Three nonpathogenic strains of Escherichia coli (BL21, DH5 alpha and K-12) were used because of easy cultivation and availability of antibodies. Several methods of antibody immobilisation were compared. The immobilisation of reduced antibody using Sulfo-SMCC was the most effective achieving the limit of detection (LOD) 10^5 CFU x mL-1. Active and passive operation modes of quartz crystal microbalance were compared concluding that for detection of microorganisms, both approaches are practically identical. The developed biosensor was connected to the cyclone air sampler allowing detection of microorganisms disseminated in the form of aerosol inside bioaerosol chamber. The achieved LOD was 1. Piezoelectric biosensors provide fast, specific and economic way of detection for a wide range of analytes ranging from metabolites to microorganisms. Specific antibodies were immobilised on gold electrodes of piezoelectric crystal and the biosensor was applied for real-time detection of model microorganisms. Three nonpathogenic strains of Escherichia coli (BL21, DH5 alpha and K-12) were used because of easy cultivation and availability of antibodies. Several methods of antibody immobilisation were compared. The immobilisation of reduced antibody using Sulfo-SMCC was the most effective achieving the limit of detection (LOD) 10^5 CFU x mL-1. Active and passive operation modes of quartz crystal microbalance were compared concluding that for detection of microorganisms, both approaches are practically identical. The developed biosensor was connected to the cyclone air sampler allowing detection of microorganisms disseminated in the form of aerosol inside bioaerosol chamber. The achieved LOD was 1. (en)
Title	Piezoelectric biosensors for detection of microorganisms Piezoelectric biosensors for detection of microorganisms (en)
skos:prefLabel	Piezoelectric biosensors for detection of microorganisms Piezoelectric biosensors for detection of microorganisms (en)
skos:notation	RIV/00216224:14740/14:00075645!RIV15-MSM-14740___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(ED1.1.00/02.0068)
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Farka, Zdeněk Skládal, Petr Kovář, David
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	36628
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/14:00075645
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Biosensor; Escherichia coli; Immunosensor; QCM (en)
http://linked.open.../riv/klicoveSlovo	Immunosensor Escherichia coli QCM Biosensor
http://linked.open...ontrolniKodProRIV	[03DABA56B6CD]
http://linked.open...v/mistoKonaniAkce	Brno
http://linked.open...i/riv/mistoVydani	Brno
http://linked.open...i/riv/nazevZdroje	XIV. Pracovní setkání fyzikálních chemiků a elektrochemiků
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Central european institute of technology
http://linked.open...UplatneniVysledku	2014
http://linked.open...iv/tvurceVysledku	Skládal, Petr Kovář, David Farka, Zdeněk
http://linked.open...vavai/riv/typAkce	CST - Celostátní
http://linked.open.../riv/zahajeniAkce	2014-06-03 (xsd:date)
number of pages	4 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Masarykova univerzita
https://schema.org/isbn	9788021068421
http://localhost/t...ganizacniJednotka	14740

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