About: Single-Base Resolution Sequence-Directed Nucleosome Mapping

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://onlinelibrary.wiley.com/doi/10.1002/ijch.201200074/abstract
Description	Frequently used nucleosome mapping techniques, both experimental and computational ones, pursue a rather simple task to determine whether a given sequence segment is likely to belong to a nucleosome, thus measuring the nucleosome occupancy along the sequence. A more ambitious task is to determine the position with high resolution, so that not only the approximate translational position of the nucleosome on DNA would be known, but also the rotational setting of the DNA. The rotational setting is important to know since the binding of various transcription factors to the nucleosome DNA crucially depends on the accessibility of the respective recognition sequences. The only experimental technique that provides the highest possible accuracy of the positioning is crystallization of the nucleosomes reconstituted on specific sequences, with subsequent solving of their structures from x-ray diffraction data. Frequently used nucleosome mapping techniques, both experimental and computational ones, pursue a rather simple task to determine whether a given sequence segment is likely to belong to a nucleosome, thus measuring the nucleosome occupancy along the sequence. A more ambitious task is to determine the position with high resolution, so that not only the approximate translational position of the nucleosome on DNA would be known, but also the rotational setting of the DNA. The rotational setting is important to know since the binding of various transcription factors to the nucleosome DNA crucially depends on the accessibility of the respective recognition sequences. The only experimental technique that provides the highest possible accuracy of the positioning is crystallization of the nucleosomes reconstituted on specific sequences, with subsequent solving of their structures from x-ray diffraction data. (en)
Title	Single-Base Resolution Sequence-Directed Nucleosome Mapping Single-Base Resolution Sequence-Directed Nucleosome Mapping (en)
skos:prefLabel	Single-Base Resolution Sequence-Directed Nucleosome Mapping Single-Base Resolution Sequence-Directed Nucleosome Mapping (en)
skos:notation	RIV/00216224:14740/13:00069571!RIV14-MSM-14740___
http://linked.open...avai/predkladatel	Středoevropský technologický institut
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM0021622415)
http://linked.open...iv/cisloPeriodika	3-4
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Hapala, Jan Trifonov, Eduard Nikolajevič
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	105336
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/13:00069571
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DNA; ucleobases; nucleosome positioning; regulation of transcription; TATA box (en)
http://linked.open.../riv/klicoveSlovo	regulation of transcription ucleobases DNA nucleosome positioning TATA box
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[FA6911294B2C]
http://linked.open...i/riv/nazevZdroje	Israel Journal of Chemistry
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	53
http://linked.open...iv/tvurceVysledku	Hapala, Jan Trifonov, Eduard Nikolajevič
http://linked.open...ain/vavai/riv/wos	000317859800003
http://linked.open...n/vavai/riv/zamer	Molecular basis of cell and tissue regulations
issn	0021-2148
number of pages	3 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/ijch.201200074
http://localhost/t...ganizacniJednotka	14740

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