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Description
  • Enzymatic reactor based on the powder of silver solid amalgam was suggested as the main part of biosensors in flow systems for the first time. 4-aminothiophenol, glutaraldehyde and enzyme were gradually bonded to the amalgam surface. Large surface of the fine amalgam particles maintains a big quantity of enzyme molecules. Biosensors were tested with enzymes ascorbate oxidase, glucose oxidase, catalase, tyrosinase and laccase. Electrochemical detection of the oxygen concentration change (for the first 3 enzymes) or the quinones concentration (tyrosinase and laccase) in the measured solution was done amperometrically by means of the tubular detector of silver solid amalgam. The current response of each biosensor was optimized with respect to the detection potential, the flow rate of the mobile phase, the injection volume and the enzymatic reactor volume. Under the found optimum conditions, concentration dependence and some statistical parameters of repeated measurements (relative standard deviation (RSD) for the studied enzymes was in the range 0.81 - 2.1 %) were measured. Biosensor with the ascorbate oxidase reactor was used for determination of ascorbic acid in the vitamin tablet Celaskon(R). Results of the analysis were in good agreement with the contents of ascorbic acid declared by manufacturer and the RSD of these analyses was 2.0 %. (en)
  • Elektrochemický enzymatický reaktor s práškovým amalgámem byl navržen jako hlavní část biosenzoru pro průtokové systémy.
  • Elektrochemický enzymatický reaktor s práškovým amalgámem byl navržen jako hlavní část biosenzoru pro průtokové systémy. (cs)
Title
  • Electrochemical Biosensors Based on Enzymatic Reactor with Amalgam Powder (en)
  • Elektrochemické enzymatické biosenzory na základě reaktoru s práškovým amalgámem
  • Elektrochemické enzymatické biosenzory na základě reaktoru s práškovým amalgámem (cs)
skos:prefLabel
  • Electrochemical Biosensors Based on Enzymatic Reactor with Amalgam Powder (en)
  • Elektrochemické enzymatické biosenzory na základě reaktoru s práškovým amalgámem
  • Elektrochemické enzymatické biosenzory na základě reaktoru s práškovým amalgámem (cs)
skos:notation
  • RIV/00216208:11310/14:10285804!RIV15-MSM-11310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • I, S
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 14247
http://linked.open...ai/riv/idVysledku
  • RIV/00216208:11310/14:10285804
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Powdered enzymatic reactor; Solid amalgam; Amperometry; Flow analysis; Electrochemical biosensors (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [334CD7892ED1]
http://linked.open...v/mistoKonaniAkce
  • Jetřichovice, Czech Republic
http://linked.open...i/riv/mistoVydani
  • Ústí nad Labem
http://linked.open...i/riv/nazevZdroje
  • Sborník přednášek mezinárodní odborné konference XXXIV. Moderní Elektrochemické Metody Jetřichovice 19.- 23. května 2014
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Barek, Jiří
  • Josypčuk, Bohdan
  • Josypčuk, Oksana
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
number of pages
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  • Srsenová Lenka - Best servis Ústí nad Labem
https://schema.org/isbn
  • 978-80-905221-2-1
http://localhost/t...ganizacniJednotka
  • 11310
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