About: Molecular characterization of a porcine sapovirus strain isolated from a piglet with diarrhoea: a case report

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About: Molecular characterization of a porcine sapovirus strain isolated from a piglet with diarrhoea: a case report Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Porcine sapoviruse have been considered as an aethiological agent of gastroenteritis in pigs. In this study, we therefore analysed 251 faecal samples obtained from diarrhoeic pigs in the Czech Republic during January 2005-June 2010 and tested them by negative staining electron microscopy for the presence of sapoviruses. Only one sample showed the presence of viral particles with characteristic sapovirus morphology. Presence of sapovirus was confirmed by RT-PCR assay with primers specific to sapoviral RNA polymerase gene and capsid gene. The phylogenetic analysis based on partial RNA polymerase sequence placed the new Czech isolate into the GVII genogroup of porcine sapoviruses, however, analysis of partial capsid gene sequence classified isolate as GIII of the genus Sapovirus. The findings of phylogenetic analysis indicate, that recombinant porcine sapovirus was identified. According to our knowledge this is the first description of porcine sapovirus in domestic pigs in the Czech Republic. Porcine sapoviruse have been considered as an aethiological agent of gastroenteritis in pigs. In this study, we therefore analysed 251 faecal samples obtained from diarrhoeic pigs in the Czech Republic during January 2005-June 2010 and tested them by negative staining electron microscopy for the presence of sapoviruses. Only one sample showed the presence of viral particles with characteristic sapovirus morphology. Presence of sapovirus was confirmed by RT-PCR assay with primers specific to sapoviral RNA polymerase gene and capsid gene. The phylogenetic analysis based on partial RNA polymerase sequence placed the new Czech isolate into the GVII genogroup of porcine sapoviruses, however, analysis of partial capsid gene sequence classified isolate as GIII of the genus Sapovirus. The findings of phylogenetic analysis indicate, that recombinant porcine sapovirus was identified. According to our knowledge this is the first description of porcine sapovirus in domestic pigs in the Czech Republic. (en)
Title	Molecular characterization of a porcine sapovirus strain isolated from a piglet with diarrhoea: a case report Molecular characterization of a porcine sapovirus strain isolated from a piglet with diarrhoea: a case report (en)
skos:prefLabel	Molecular characterization of a porcine sapovirus strain isolated from a piglet with diarrhoea: a case report Molecular characterization of a porcine sapovirus strain isolated from a piglet with diarrhoea: a case report (en)
skos:notation	RIV/00027162:_____/11:#0000770!RIV12-GA0-00027162
http://linked.open...avai/predkladatel	Výzkumný ústav veterinárního lékařství, v.v.i,
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(ED0006/01/01), P(GP524/09/P195)
http://linked.open...iv/cisloPeriodika	8
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Kulich, Pavel Dufková, Lucie Prodělalová, Jana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	213479
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/11:#0000770
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	calicivirus; RT-PCR; phylogenetic analysis; genogroup; enteritis (en)
http://linked.open.../riv/klicoveSlovo	calicivirus enteritis genogroup phylogenetic analysis RT-PCR
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[733E27B6C98D]
http://linked.open...i/riv/nazevZdroje	Veterinární Medicína
http://linked.open...in/vavai/riv/obor	GJ
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Detection and characterization of porcine teschoviruses and enteroviruses in pig breeding in the Czech Republic Center for Advanced Microbiology & Immunology Research in Veterinary Medicine
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	56
http://linked.open...iv/tvurceVysledku	Kulich, Pavel Dufková, Lucie Prodělalová, Jana
http://linked.open...ain/vavai/riv/wos	000295252600007
issn	0375-8427
number of pages	7 (xsd:int)

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