About: The use of bioinfomatics to retrieve sequencing data of wheat and barley polymorphic DNAs for production of reliable DNA markers     Goto   Sponge   NotDistinct   Permalink

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Description
  • The main objective of the project is to use and improve selected statistical and bioinformatics tools in order to produce, analyze, and integrate high-throughput genomic sequence data. This will allow understand better the biological systems in plants. Sequencing analysis is the most precise procedure that allows obtaining information on relation between the gene primary structure and corresponding phenotypic trait, which namely important in case of agricultural important crop species. We aim to employ available data on polymoprhisms of coding sequences in wheat to develop DNA markers derived from coding sequences associated with phenotypic traits. Such markers can be used directly for marker assisted selection and can serve as genotype/cultivar characteristics. Available data are incomplete and suffer from inaccuracy. More over wheat (T. aestivum L.) has a large genome (hexaploid) with over 90% of noncoding sequenced including pseudogenes. Within the project we intend to (1) analyze available data (en)
  • Cílem projektu je využít potenciál mezináordního kolektivu a aplikovat a zlepšit nástroje bioinformatiky pro analýzu sekvencí rostliného genomu a vývoj DNA markérů. Získat nové informace o sekvenační variabilitě alel a nové postupy na nich validovat.
Title
  • The use of bioinfomatics to retrieve sequencing data of wheat and barley polymorphic DNAs for production of reliable DNA markers (en)
  • Využití nástrojů bioinformatiky pro hodnocení sekvenčních dat pšenice a ječmene pro vývoj spolehlivých molekulárních markerů.
skos:notation
  • OC10017
http://linked.open...avai/cep/aktivita
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http://linked.open...ep/duvernostUdaju
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  • bioinformatic; DNA sequences; barley; wheat; molecular marker (en)
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  • The project was focused on the evaluation, use and application of bioinformatics tools for the analysis and use of sequencing and transkriptomic data, molecular markers. We focused on the use of Ginni-Simpson index and Bayesian probabilistics. Data files were analyzed and obtained the original data. Information about the poymorphic DNA sequences were obtained. HRM based markers were introduced. (en)
  • Projekt byl zaměřen na hodnocení, využití a aplikaci nástrojů bioinformatiky pro analýzu a využití sekvenačních a transkriptomickéch dat, molekulární markérů. Zaměřili jsme se na využit Ginni-simpsonova indexu a bayesovské probabilistiky. Byly analyzovány datové soubory a byla získána originální data. Byly získány informace o sekvencích DNA. Byly zavedeny markéry na bázi HRM. (cs)
http://linked.open...tniCyklusProjektu
http://linked.open.../cep/klicoveSlovo
  • DNA sequences
  • barley
  • wheat
  • bioinformatic
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