About: Solvent relaxtion in phospholipid bilayers: physical understanding and biophysical applications     Goto   Sponge   NotDistinct   Permalink

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  • After the uderstanding of solvent relaxation (SR) in isotropic solvents, scinetists started abour 10 years ago to characterise SR in biosasemblies. It was our group that established the SR method for probing micro-mobilities and -polarities in biomembranes. Despite several applications of the SR method in membrane research, the origin of the observed slow SR kinetics probed by dyes defined localised in the external interface, headgroup region, or backbone region of the bilayer is still unclear. We suggest to combine defined fluorescence experiments with molecular dynamic simulations in order to elucidate the origin of the nanosecond SR kinetics observed in the headgroup and backbone region of lipid bilayers. The second part of the proposal comprises an application of the SR approach in an actual question in protein sciences, the characterisation of the interaction of the alfa-defensine cryptidin-4 with membranes in order to understand the bactericidal properties of this protein. (en)
  • Po uspokojivém výkladui relaxace rozpouštědla (RR) v isotropních rozpouštědlech byla před 10 lety započata charakterizace RR v biomakromolekulách. Naše skupina zavedla metodu RR pro sledování micro-mobility a -polarity v biomembránách. I přes použití této metody ve výzkumu biomembrán, fyzikální původ pozorované %22pomalé%22 kinetiky RR, monitorované pomocí definovaně lokalizovaných fluorescenčních sond v celém profilu dvojvrstvy, není dosud zcela jasný. Navrhujeme proto spojit experimenty se simulacemi molekulární dynamiky, abychom objasnili nanosekundovou kinetiku pozorovanou v oblasti polárních hlavic a nepolárních řetězců ve fosfolipidové dvojvrstvě. Druhá část projektu zahrnuje aplikaci metody RR ve výzkumu proteinů, a to charakterizaci interakce alfa-defensinu cryptinu-4 s biomembránami za účelem porozumění baktericidních vlastností tohoto proteinu.
Title
  • Solvent relaxtion in phospholipid bilayers: physical understanding and biophysical applications (en)
  • Relaxace rozpouštědla ve fosfolipidových dvojvrstvách: fyzikální výklad a biofyzikální aplikace
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  • IAA400400503
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  • time-resolved fluorescence; dipolar solvent relaxation; Stokes-shift; molecular dynamic simulations; lipid headgroup; lipid backbone; n-AS dyes; Patman; photophysics; PEGylated lipids; DOPC; defensine; cryptdin; biological water; FCS; ellipsometry (en)
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  • Combination of fluorescence experiments and theoretical chemistry led to molecular interpretation of time-dependent Stokes shifts of relevant membrane markers. Applications of these methods on actual biological systems were published. (en)
  • Kombinace fluorescenčních experimentů a teoretické chemie vedla k molekularní interpretaci časově závislých Stokesovských posunů pro relevantní membránové fluorescenční značky. Byly publikovány aplikace těchto metod na aktuální biologické systémy. (cs)
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  • time-resolved fluorescence
  • DOPC
  • FCS
  • PEGylated lipids
  • Patman
  • Stokes-shift
  • biological water
  • cryptdin
  • defensine
  • dipolar solvent relaxation
  • lipid backbone
  • lipid headgroup
  • molecular dynamic simulations
  • n-AS dyes
  • photophysics
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