About: Cell culture for propagation of prions: influence of cell culture proliferation and expression of endogenous retroviruses     Goto   Sponge   NotDistinct   Permalink

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Description
  • The unifying pathological mechanism of Transmissible spongifom encephalopathies or prion diseases, is the misfolding of cellular prion protein - PrPc into infectious isoform - PrPtse. Little is known about physiological function of PrPc and also processes of conformational changes from PrPc to the PrPtse.Cell lines are convenient experimental systems for analyzing the molecular and cellular mechanisms of prion propagation. Prion propagation in cell culture differs fundamentally from the course of infection in vivo. Conditions in vitro are unique for the study of interplay between de novo prion formation, catabolism, cell division, and horizontal transmission. Although PrPc is essential for the development of prion diseases other factors are thought to be involved in the PrPtse formation and replication. One of these factors could be retroviruses respectively endogenous retroviruses.Investigation of some aspects of cell proliferation for the infection and propagation of prions and significance of expression of endogenous retroviruses in infected cells is aim of the present proposal. (en)
  • Cíle: 1.Zjištění vnímavosti buněčných linií k infekci PrPtse v závislosti na buněčné dělení. 2.Zjištění propagace PrPtse v závislosti na buněčném dělení a sledování mechanismu přenosu infekce. 3.Sledování exprese endogenních retrovirů v buněčných liniích infikovaných různými kmeny PrPtse.
Title
  • Cell culture for propagation of prions: influence of cell culture proliferation and expression of endogenous retroviruses (en)
  • Propagace prionů v buněčných kulturách: vliv podmínek kultivace buněčných linií a exprese endogenních retrovirů
skos:notation
  • GP310/09/P260
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  • buněčné kultury, priony, endogenní retroviry, propagace a šíření prionů (en)
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  • The project was excellent, both in terms of expertise and in terms of disbursement of funds. (en)
  • Řešení projektu proběhlo na vynikající úrovni, a to jak z hlediska odborného tak i z hlediska čerpání finančních prostředků. (cs)
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  • endogenní retroviry
  • priony
  • buněčné kultury
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