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  • Bladder cancer is the second most frequent malignancy among the tumours of the urinary tract in men - prostate carcinoma being the first. Extensive heterogeneity in tumour behaviour and the absence of accurate method for the early detection of tumour progression or relapse lead to the intensive search for new prognostic markers. The aim of the proposed project is to verify the possible use of p16 gene as a prognostic marker for the follow-up of patients with bladder transitional cell carcinomas. We will establish very sensitive and exact approach: real-time quantitative polymerase chain reaction (RQ-PCR). We will compare p16 status (normal, hemizygous deletion, homozygous deletion) in a normal patient's tissue, tumour and urine specimen. We will trto define the relationship between this potential marker and histopathological and clinical features of the tumour as well as continuous tumour progression and/or relapse in the cohort of patients. Moreover, we will test DNA from the urine sediment as a (en)
  • N dory moźov‚ho mŘchěýe jsou po karcinomu prostaty druhěm nejźastŘjçˇm urologickěm novotvarem u mu§…. Znaźn  heterogenita v chov nˇ n dor… a absence spolehliv‚ metody pro vźasnou detekci n dorov‚ progrese źi recidivy vedou k intensivnˇmu hled nˇ nověch prognostickěch znak…. Cˇlem navrhovan‚ho projektu je ovŘýit mo§nost vyu§itˇ n dorov‚ho supresorov‚ho genu p16 jako prognostick‚ho markeru pýi sledov nˇ pacient… s uroteli lnˇmi n dory moźov‚ho mŘchěýe. Bude zavedena kvantitativnˇ polymerasov  ýetŘzov  reakce v re ln‚m źase (RQ-PCR), velice citliv  a pýesn  metoda pro kvantifikaci gen…. Status genu p16 (norm lnˇ stav, hemizygotnˇ nebo homozygotnˇ delece) bude porovn v n mezi norm lnˇ tk nˇ, n dorovou tk nˇ a vzorkem moźi konkr‚tnˇho pacienta. D…le§itousouź stˇ projektu bude definov nˇ vztah… tohoto mo§n‚ho markeru jak k histopatologickěm a klinickěm parametr…m tumoru, tak i k dalçˇ progresi źi recidivŘ n doru sledovaněch pacient…. D le budeme testovat DNA moźov‚ho sedimentu jako mo§ně zdroj informacˇ
Title
  • Quantitative analysis of p16 gene as a potential prognostic marker of bladder transitional cell carcinomas (en)
  • Kvantitativnˇ stanovenˇ genu p16 jako mo§n‚ho prognostick‚ho markeru u uroteli lnˇch n dor… moźov‚ho mŘchěýe
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  • GP301/01/D142
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  • Projekt byl pýedźasnŘ ukonźen na § dost ýeçitelky, vzhledem k n stupu na mateýskou dovolenou. Cˇlem projektu bylo vyu§ˇt alelickěch forem genu p 16 pro diagnostiku n dor… moźov‚ho mŘchěýe. Jediněm pýˇnosem projektu bylo shrom §dŘnˇ klinick‚ho materi lu p (cs)
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