About: Combination of molecular genetic and imunochemical principles for specific and rapid detection of Enterobacter sakazakii in foods     Goto   Sponge   NotDistinct   Permalink

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Description
  • SummaryEnterobacter sakazakii is a pathogen causing disease in premature babies and newborn infants, rarely in adults. Despite the low frequency of reported infections, the high mortality rate (40-80%) has been occured. The aim of this project is to develop new, simple, rapid, specific, sensitive enough, user-friendly and cheap immunochemical techniques (enzyme immunoassay on micro plate, dipstick format, lateral flow immunoassay) as well as development of a nucleic acid lateral flow immunoassay for detection of Enterobacter sakazakii in milk and milk products. The developed methods will be verified by standard microbiological procedures. The reduced cost and improved speed of the new methods will allow much more extensive sampling than present methodology and thus also applicable in control laboratories of milk industry. It is envisaged that some of the developed methods should be incorporated into advanced quality control procedures, such as the HACCP system. (en)
  • Vyvinout a optimalizovat jednoduché, rychlé, specifické a levné metody detekce Enterobacter sakazakii v mléce a mléčných výrobcích, založené na imunochemickém a molekulárně genetickém principu a jejich kombinaci, tj. imunochromatografické detekci produktů polymerázové řetězové reakce.
Title
  • Combination of molecular genetic and imunochemical principles for specific and rapid detection of Enterobacter sakazakii in foods (en)
  • Kombinace molekulárně genetického a imunochemického principu pro specifickou a rychlou detekci Enterobacter sakazakii v potravinách
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  • GA525/09/1075
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  • enterobacter sakazakii; imunochromatografická detekce; mléko; detekce patogenů (en)
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  • The project gone according to plan both in terms of expertise and in terms of disbursement of funds. (en)
  • Řešení projektu proběhlo podle plánu jak z hlediska odborného tak i z hlediska čerpání finančních prostředků. (cs)
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  • imunochromatografická detekce
  • mléko
  • enterobacter sakazakii
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