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| rdfs:seeAlso
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| Description
| - Previously we discovered anticancerogenic effects of plant nucleases I. Antiproliferative effects were reached at protein concentrations approximately ten-times lower in comparison to studied animal RNases and side-effects were much lower than caused by animal RNases. This makes plant nucleases perspective anticancerogenic agents that could be, similarly to onconase, selected for clinical trials in the future. In the project proposed we aim to study modifications and biological properties of three cloned nucleases, TBN1 from tomato, HBN1 from hop and ABN1 from A. brassica.. Site-specific mutagenesis, cDNA shuffling and posttranslational modifications in planta are proposed to study structural-functional relationships responsible for nuclease activity, stability and for mechanism(s) of antiproliferative effects that will be analyzed in detail especially in human melanoma, and sassayed using other selected cancer models. Levels of immunosuppression, aspermatogenesis and effects on blood formation will be estimated to characterize potential side-effects of nucleases produced by in planta biotechnology. (en)
- Hlavním cílem projektu je pripravit v systému in planta standartní a modifikované varianty klonovaných bifunkčních nukleáz, vybrané alternativy analyzovat z hlediska katalytických, strukturních, antiproliferativních, biologických aktivit a mechanismu účinku u zvolených živočišných modelů.
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| Title
| - Modification and in planta production of bifunctional nucleases and analysis of their anticancerogenic and biological activities (en)
- Modifikace a produkce bifunkčních nukleáz in planta a analýza jejich protinádorových a biologických aktivit
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| skos:notation
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| http://linked.open...avai/cep/aktivita
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| http://linked.open...kovaStatniPodpora
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| http://linked.open...ep/celkoveNaklady
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| http://linked.open...datumDodatniDoRIV
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| http://linked.open...i/cep/druhSouteze
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| http://linked.open...ep/duvernostUdaju
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| http://linked.open.../cep/fazeProjektu
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| http://linked.open...ai/cep/hlavniObor
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| http://linked.open...hodnoceniProjektu
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| http://linked.open...vai/cep/kategorie
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| http://linked.open.../cep/klicovaSlova
| - plant nuclease I; N. benthamiana; leaf infiltration; A. tumefaciens; tumor cells; antiproliferative (en)
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| http://linked.open...ep/partnetrHlavni
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| http://linked.open...inujicichPrijemcu
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| http://linked.open...cep/pocetPrijemcu
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| http://linked.open...ocetSpoluPrijemcu
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| http://linked.open.../pocetVysledkuRIV
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| http://linked.open...enychVysledkuVRIV
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| http://linked.open...lneniVMinulemRoce
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| http://linked.open.../prideleniPodpory
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| http://linked.open...iciPoslednihoRoku
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| http://linked.open...atUdajeProjZameru
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| http://linked.open.../vavai/cep/soutez
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| http://linked.open...usZobrazovaneFaze
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| http://linked.open...ai/cep/typPojektu
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| http://linked.open...ep/ukonceniReseni
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| http://linked.open.../cep/vedlejsiObor
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| http://linked.open...ep/zahajeniReseni
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| http://linked.open...jektu+dodavatelem
| - The project gone according to plan both in terms of expertise and in terms of disbursement of funds. (en)
- Řešení projektu proběhlo podle plánu jak z hlediska odborného tak i z hlediska čerpání finančních prostředků. (cs)
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| http://linked.open...tniCyklusProjektu
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| http://linked.open.../cep/klicoveSlovo
| - A. tumefaciens
- N. benthamiana
- leaf infiltration
- tumor cells
- plant nuclease I
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| is http://linked.open...vavai/cep/projekt
of | |
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