About: *Development of assays for thrombosis and atherosclerosis risk mutation detection     Goto   Sponge   NotDistinct   Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Projekt, within Data Space : linked.opendata.cz associated with source document(s)

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Description
  • *Project deals with the research and developments in the field of DNA mutation detection that are linked with the thrombosis and atherosclerosis risk. The solution is based on the use of real-time PCR in combination with modified probes. The assays for detection of prevalent thromboembolic events and atherosclerosis mutations will be developed. The mutations will be in particular FV (G1691A), F II (G20210A), MTHFR (C677T), MTHFR (A1298C), MTRR (G66A) a MTR (A2756G). Detection system will be based on the allelic discrimination principle, the assay components will be the pair of specific primers and pair of modified probes. The development and mastering of the synthesis of probes containing modified bases enhancing the higher specifity and sensitivity of allelic discrimination is the key innovation coming from the project. Next step is the completation of the assays comprising primers, modified probes and control DNA. Finished product will be validated and ready for commercial evaluation. (en)
  • *Projekt řeší výzkum a vývoj v oblasti detekce mutací DNA, které mají souvislost se zvýšeným rizikem trombózy a aterosklerózy. Řešení je založeno na použití real-time PCR v kombinaci s modifikovanými sondami. Budou vyvíjeny soupravy pro detekci nejvýznamnějších mutací spojované s trombofilními stavy a aterosklerózou. Konkrétně se jedná o mutace FV (G1691A), F II (G20210A), MTHFR (C677T), MTHFR (A1298C), MTRR (G66A) a MTR (A2756G). Detekční systém bude fungovat na principu alelické diskriminace, komponentami souprav budou dvojice specifických primerů a dvojice sond (pro zdravou a mutovanou alelu). Inovací bude zvládnutí výroby hydrolyzačních sond s modifikovanými bazemi zaručujícími zvýšenou specifitu a citlivost alelické diskriminace. Dalším krokem bude kompletace detekčních systémů sestávajících z primerů a sond a z kontrolní DNA. Hotový produkt bude validován a připraven pro komerční využití.
Title
  • *Development of assays for thrombosis and atherosclerosis risk mutation detection (en)
  • *Vývoj souprav pro detekci mutací rizikových pro vznik trombózy a aterosklerózy
skos:notation
  • FR-TI1/507
http://linked.open...avai/cep/aktivita
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http://linked.open...ep/celkoveNaklady
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http://linked.open.../cep/klicovaSlova
  • mutation; molecular genetics; real-time PCR; allelic discrimination; oligonucleotide probes; atherosclerosis; thrombosis; genetic; risk (en)
http://linked.open...ep/partnetrHlavni
http://linked.open...inujicichPrijemcu
http://linked.open...cep/pocetPrijemcu
http://linked.open...ocetSpoluPrijemcu
http://linked.open.../pocetVysledkuRIV
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  • V rámci projektu byly vyvinuty čtyři soupravy pro diagnostiku trombofilních mutací založené na principu real-time PCR - Alelické diskriminace. Soupravy byly zavedeny do výroby pod normou ISO 13485, validovány a certifikovány CE IVD. (cs)
  • Four kits for diagnosis of thrombophilic mutations based on the principle of real-time PCR-Allelic discrimination were developed within the project. Kits were inproduced in to the production under ISO 13485, validated and certified by ceiva. (en)
http://linked.open...tniCyklusProjektu
http://linked.open.../cep/klicoveSlovo
  • mutation
  • allelic discrimination
  • atherosclerosis
  • genetic
  • molecular genetics
  • oligonucleotide probes
  • real-time PCR
  • thrombosis
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