About: Recondensation level of repetitive sequences in the plant protoplast nucleus is limited by oxidative stress     Goto   Sponge   NotDistinct   Permalink

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  • Protoplast cultures are remarkable examples of plant cell dedifferentiation. The state of dedifferentiation is evidenced by changes in cell morphology, genome organization, as well as by the capability of protoplasts to differentiate into multiple types of cells. The first change in the genome structure is connected with large-scale chromatin decondensation, affecting chromocenters involving various types of these repetitive sequences. This paper describes not only the de- and re-condensation of repetitive sequences, but it also compares the recondensation level of chromatin with the levels of oxidative stress which were decreased by using an antioxidant. We have demonstrated that the treatment of protoplasts with ascorbic acid not only decreased the level of oxidative stress but also positively stimulated the expression of the ascorbate peroxidase and catalase. It also led to a greater recondensation of the chromatin (when compared to the untreated protoplasts).
  • Protoplast cultures are remarkable examples of plant cell dedifferentiation. The state of dedifferentiation is evidenced by changes in cell morphology, genome organization, as well as by the capability of protoplasts to differentiate into multiple types of cells. The first change in the genome structure is connected with large-scale chromatin decondensation, affecting chromocenters involving various types of these repetitive sequences. This paper describes not only the de- and re-condensation of repetitive sequences, but it also compares the recondensation level of chromatin with the levels of oxidative stress which were decreased by using an antioxidant. We have demonstrated that the treatment of protoplasts with ascorbic acid not only decreased the level of oxidative stress but also positively stimulated the expression of the ascorbate peroxidase and catalase. It also led to a greater recondensation of the chromatin (when compared to the untreated protoplasts). (en)
Title
  • Recondensation level of repetitive sequences in the plant protoplast nucleus is limited by oxidative stress
  • Recondensation level of repetitive sequences in the plant protoplast nucleus is limited by oxidative stress (en)
skos:prefLabel
  • Recondensation level of repetitive sequences in the plant protoplast nucleus is limited by oxidative stress
  • Recondensation level of repetitive sequences in the plant protoplast nucleus is limited by oxidative stress (en)
skos:notation
  • RIV/61989592:15310/10:10212711!RIV11-MSM-15310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(2E08018), Z(MSM6198959215)
http://linked.open...iv/cisloPeriodika
  • 9
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 284206
http://linked.open...ai/riv/idVysledku
  • RIV/61989592:15310/10:10212711
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • protoplasts; oxidative stress; nuclear organization; chromatin (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [8DBDAE9EE3C1]
http://linked.open...i/riv/nazevZdroje
  • Journal of Experimenal Botany
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 61
http://linked.open...iv/tvurceVysledku
  • Lebeda, Aleš
  • Navrátilová, Božena
  • Ondřej, Vladan
  • Sedlářová, Michaela
  • Luhová, Lenka
  • Piterková, Jana
  • Protivánková, Iva
http://linked.open...ain/vavai/riv/wos
  • 000279003600015
http://linked.open...n/vavai/riv/zamer
issn
  • 0022-0957
number of pages
http://localhost/t...ganizacniJednotka
  • 15310
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