About: The heterochromatin as a marker for protoplast differentiation of Cucumis sativus     Goto   Sponge   NotDistinct   Permalink

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  • The protoplast cultures of Cucumis sativus in two culture systems were used to study heterochromatin reassembly during dedifferentiation of isolated protoplasts and their subsequent differentiation into calli and proembryos. Here we show that dedifferantiation of the cucumber mesophyll cells is accompanied by a dramatic reduction in size and numbers of nuclear chromocenters. Although chromocenters were newly established during protoplast culture, the measured relative heterochromatin content differed according to the culture system used. Protoplast culture leading to proembryo formation displayed a lower level of relative heterochromatin content than cultures resulting in calli and the relative heterochromatin content reached values close to those estimated for somatic embryos.
  • The protoplast cultures of Cucumis sativus in two culture systems were used to study heterochromatin reassembly during dedifferentiation of isolated protoplasts and their subsequent differentiation into calli and proembryos. Here we show that dedifferantiation of the cucumber mesophyll cells is accompanied by a dramatic reduction in size and numbers of nuclear chromocenters. Although chromocenters were newly established during protoplast culture, the measured relative heterochromatin content differed according to the culture system used. Protoplast culture leading to proembryo formation displayed a lower level of relative heterochromatin content than cultures resulting in calli and the relative heterochromatin content reached values close to those estimated for somatic embryos. (en)
Title
  • The heterochromatin as a marker for protoplast differentiation of Cucumis sativus
  • The heterochromatin as a marker for protoplast differentiation of Cucumis sativus (en)
skos:prefLabel
  • The heterochromatin as a marker for protoplast differentiation of Cucumis sativus
  • The heterochromatin as a marker for protoplast differentiation of Cucumis sativus (en)
skos:notation
  • RIV/61989592:15310/09:00010196!RIV10-MSM-15310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM6198959215)
http://linked.open...iv/cisloPeriodika
  • 2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 370004
http://linked.open...ai/riv/idVysledku
  • RIV/61989592:15310/09:00010196
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Cucumber; Protoplasts; Callus; Proembryos; Nuclear chromocenters; Heterochromatin decondensation (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [7A130C222AB6]
http://linked.open...i/riv/nazevZdroje
  • Plant Cell, Tissue and Organ Culture
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 96
http://linked.open...iv/tvurceVysledku
  • Lebeda, Aleš
  • Navrátilová, Božena
  • Ondřej, Vladan
http://linked.open...ain/vavai/riv/wos
  • 000262088700013
http://linked.open...n/vavai/riv/zamer
issn
  • 1573-5044
number of pages
http://localhost/t...ganizacniJednotka
  • 15310
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