About: Chromatin Structural Rearrangement during Dedifferentiation of Protoplasts of Cucumis sativus L.

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About: Chromatin Structural Rearrangement during Dedifferentiation of Protoplasts of Cucumis sativus L. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	This paper reports on the structural rearrangement of satellite DNA type I repeats and heterochromatin during the dedifferentiation and cell cycling of mesophyll protoplasts of cucumber (Cucumis sativus). These repeats were localized in the telomeric heterochromatin of cucumber chromosomes and in the chromocenters of interphase nuclei. The dramatic reduction of heterochromatin involves decondensation of subtelomeric repeats in freshly isolated protoplasts; however, there are not a great many remarkable changes in the expression profile. In spite of that, reformation of the chromocenters, occurring 48 h after protoplast isolation, is accompanied by recondensation of satellite DNA type I; however, only partial reassembly of these repeats was revealed. In this study, FISH and a flow cytometry assay show a correlation between the partial chromocenter and the repeats reassembly, and with the reentry of cultivated protoplasts into the cell cycle and first cell division. After that, divided cells displayed a This paper reports on the structural rearrangement of satellite DNA type I repeats and heterochromatin during the dedifferentiation and cell cycling of mesophyll protoplasts of cucumber (Cucumis sativus). These repeats were localized in the telomeric heterochromatin of cucumber chromosomes and in the chromocenters of interphase nuclei. The dramatic reduction of heterochromatin involves decondensation of subtelomeric repeats in freshly isolated protoplasts; however, there are not a great many remarkable changes in the expression profile. In spite of that, reformation of the chromocenters, occurring 48 h after protoplast isolation, is accompanied by recondensation of satellite DNA type I; however, only partial reassembly of these repeats was revealed. In this study, FISH and a flow cytometry assay show a correlation between the partial chromocenter and the repeats reassembly, and with the reentry of cultivated protoplasts into the cell cycle and first cell division. After that, divided cells displayed a (en)
Title	Chromatin Structural Rearrangement during Dedifferentiation of Protoplasts of Cucumis sativus L. Chromatin Structural Rearrangement during Dedifferentiation of Protoplasts of Cucumis sativus L. (en)
skos:prefLabel	Chromatin Structural Rearrangement during Dedifferentiation of Protoplasts of Cucumis sativus L. Chromatin Structural Rearrangement during Dedifferentiation of Protoplasts of Cucumis sativus L. (en)
skos:notation	RIV/61989592:15310/09:00010056!RIV10-MSM-15310___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM6198959215)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Nádvorník, Petr Navrátilová, Božena Ondřej, Vladan Doležalová, Ivana Lebeda, Aleš Kitner, Miloslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	307038
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/09:00010056
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cell cycle; chromatin; plant protoplasts; subtelomeric repeats (en)
http://linked.open.../riv/klicoveSlovo	chromatin cell cycle plant protoplasts subtelomeric repeats
http://linked.open...odStatuVydavatele	KR - Korejská republika
http://linked.open...ontrolniKodProRIV	[7116102A5279]
http://linked.open...i/riv/nazevZdroje	Molecules and Cells
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	27
http://linked.open...iv/tvurceVysledku	Kitner, Miloslav Lebeda, Aleš Navrátilová, Božena Ondřej, Vladan Doležalová, Ivana Nádvorník, Petr
http://linked.open...n/vavai/riv/zamer	Variabilita složek a interakcí v rostlinném patosystému a vliv faktorů prostředí na jejich projev
issn	1016-8478
number of pages	5 (xsd:int)
http://localhost/t...ganizacniJednotka	15310

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