About: (Strept)avidin-biotin interactions at amalgam electrodes covered by thiol monolayer

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Carboxylic group of 11-mercaptoundecanoic acid (MUA) can be used to creat a peptide bond with species containing amino group, e.g., peptides, and proteins. By the help of EDC-NHS technology, streptavidin or avidin was covalently bonded with MUA-monolayer at a silver solid amalgam electrode. Such prepared electrode was used for detecting biotin and biotinylated albumin in the supporting electrolyte (0.15 M NaCl, 0.05 M TRIS, pH 7.0). Electrochemical impendance spectroscopy was performed for the biosensor response monitored by impedance spectroscopy. Binding of biotin or biotinylated albumin with (strept)avidin a change in the resistance of the sensor in the concentration range of 0.5-20 μg mL-1. Electrochemical regeneration of the amalgam electrode permits simply to renew its surface and to create the new biosensor. Carboxylic group of 11-mercaptoundecanoic acid (MUA) can be used to creat a peptide bond with species containing amino group, e.g., peptides, and proteins. By the help of EDC-NHS technology, streptavidin or avidin was covalently bonded with MUA-monolayer at a silver solid amalgam electrode. Such prepared electrode was used for detecting biotin and biotinylated albumin in the supporting electrolyte (0.15 M NaCl, 0.05 M TRIS, pH 7.0). Electrochemical impendance spectroscopy was performed for the biosensor response monitored by impedance spectroscopy. Binding of biotin or biotinylated albumin with (strept)avidin a change in the resistance of the sensor in the concentration range of 0.5-20 μg mL-1. Electrochemical regeneration of the amalgam electrode permits simply to renew its surface and to create the new biosensor. (en)
Title	(Strept)avidin-biotin interactions at amalgam electrodes covered by thiol monolayer (Strept)avidin-biotin interactions at amalgam electrodes covered by thiol monolayer (en)
skos:prefLabel	(Strept)avidin-biotin interactions at amalgam electrodes covered by thiol monolayer (Strept)avidin-biotin interactions at amalgam electrodes covered by thiol monolayer (en)
skos:notation	RIV/61388955:_____/12:00377444!RIV13-GA0-61388955
http://linked.open...avai/riv/aktivita	I P S Z
http://linked.open...avai/riv/aktivity	I, P(GAP206/11/1638), P(GAP208/12/1645), P(IAA400400806), S, Z(MSM0021620857)
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Mareček, Vladimír Yosypchuk, Bogdan
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav fyzikální chemie J. Heyrovského AV ČR, v.v.i.
http://linked.open...dnocenehoVysledku	171681
http://linked.open...ai/riv/idVysledku	RIV/61388955:_____/12:00377444
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	voltammetry; amalgam electrodes; monolayer (en)
http://linked.open.../riv/klicoveSlovo	amalgam electrodes voltammetry monolayer
http://linked.open...ontrolniKodProRIV	[4C41DC4370AF]
http://linked.open...v/mistoKonaniAkce	Jetřichovice
http://linked.open...i/riv/mistoVydani	Ústí nad Labem
http://linked.open...i/riv/nazevZdroje	XXXII. Moderní elektrochemické metody. Sborník přednášek
http://linked.open...in/vavai/riv/obor	CG
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Novel electrochemical sensors and sensing techniques for the analysis of nucleic acids structure and interactions Transport of Charged and Uncharged Species across Models of Biological Membranes Aspects of hazardous metals transfer across biomembranes
http://linked.open...UplatneniVysledku	2012
http://linked.open...iv/tvurceVysledku	Yosypchuk, Bogdan Yosypchuk, O. Mareček, Vladimír
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2012-05-21 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Nové molekulární systémy pro pokročilé aplikace prospěšné pro zdraví a šetrné k životnímu prostředí
number of pages	4 (xsd:int)
http://purl.org/ne...btex#hasPublisher	BEST servis
https://schema.org/isbn	978-80-905221-0-7

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