About: Bioinformatic and image analyses of the cellular localization of the apoptotic proteins endonuclease G, AIF, and AMID during apoptosis in human cells

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About: Bioinformatic and image analyses of the cellular localization of the apoptotic proteins endonuclease G, AIF, and AMID during apoptosis in human cells Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We studied the cellular localization of the apoptotic proteins endonuclease G, AIF, and AMID in silico using three prediction tools and in living cells using both single-cell colocalization image analysis and nuclear translocation analysis. We confirmed the mitochondrial localization of endonuclease G and AIF by prediction analysis and by single-cell colocalization image analysis. We found the AMID protein to be cytoplasmic, most probably incorporated into the cytoplasmic side of the membranes of various organelles. The highest concentration of AMID was observed associated with the Golgi. Colocalization of AMID with lysosomes was also indirectly confirmed by analysis of AMID-rich vesicle velocity using manual tracking analysis. Bioinformatic analysis also detected nuclear localization signals in endonuclease G and AIF, but not in AMID. A novel analysis of time-lapse fluorescence image data during staurosporine-induced apoptosis revealed nuclear translocation only for endonuclease G and AIF. We studied the cellular localization of the apoptotic proteins endonuclease G, AIF, and AMID in silico using three prediction tools and in living cells using both single-cell colocalization image analysis and nuclear translocation analysis. We confirmed the mitochondrial localization of endonuclease G and AIF by prediction analysis and by single-cell colocalization image analysis. We found the AMID protein to be cytoplasmic, most probably incorporated into the cytoplasmic side of the membranes of various organelles. The highest concentration of AMID was observed associated with the Golgi. Colocalization of AMID with lysosomes was also indirectly confirmed by analysis of AMID-rich vesicle velocity using manual tracking analysis. Bioinformatic analysis also detected nuclear localization signals in endonuclease G and AIF, but not in AMID. A novel analysis of time-lapse fluorescence image data during staurosporine-induced apoptosis revealed nuclear translocation only for endonuclease G and AIF. (en)
Title	Bioinformatic and image analyses of the cellular localization of the apoptotic proteins endonuclease G, AIF, and AMID during apoptosis in human cells Bioinformatic and image analyses of the cellular localization of the apoptotic proteins endonuclease G, AIF, and AMID during apoptosis in human cells (en)
skos:prefLabel	Bioinformatic and image analyses of the cellular localization of the apoptotic proteins endonuclease G, AIF, and AMID during apoptosis in human cells Bioinformatic and image analyses of the cellular localization of the apoptotic proteins endonuclease G, AIF, and AMID during apoptosis in human cells (en)
skos:notation	RIV/00216224:14330/07:00020060!RIV10-GA0-14330___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1P05OC084), P(1QS500040508), P(GA202/04/0907), P(GP204/03/D031), P(GP204/05/P090), P(LC535), Z(AV0Z50040507), Z(AV0Z50040702), Z(MSM0021622419)
http://linked.open...iv/cisloPeriodika	7
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Amrichová, Jana Ulman, Vladimír Vařecha, Miroslav Kozubek, Michal Zimmermann, Michal Lukášová, Emilie
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Fakulta informatiky
http://linked.open...dnocenehoVysledku	411848
http://linked.open...ai/riv/idVysledku	RIV/00216224:14330/07:00020060
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	apoptosis-inducing factor; endonuclease G; AIF-homologous mitochondrion-associated inducer of death; living cells; colocalization image analysis; analysis of amino acid sequence (en)
http://linked.open.../riv/klicoveSlovo	apoptosis-inducing factor endonuclease G living cells analysis of amino acid sequence colocalization image analysis AIF-homologous mitochondrion-associated inducer of death
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[3C2B743AD858]
http://linked.open...i/riv/nazevZdroje	Apoptosis
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Dynamic structure and function of the cell nucleus after irradiation Methylation of histone H3 as a prognostic marker of chronic myeloid leukemia remission Chromosome dynamics and organization during the cell cycle in normal and pathologic conditions High-resolution cytomery of living cells Apoptosis-inducing factor (AIF): its translocation from mitochondria and its action on nuclear chromatin Topographic and functional characterization of telomeres in various tumor cell lines determined in situ and in vivo
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	12
http://linked.open...iv/tvurceVysledku	Lukášová, Emilie Ulman, Vladimír Kozubek, Michal Vařecha, Miroslav Zimmermann, Michal Amrichová, Jana
http://linked.open...ain/vavai/riv/wos	000247184000003
http://linked.open...n/vavai/riv/zamer	Biophysics of dynamic structures and functions of biological systems Genome and epigenome: 1D and 3D Structure, Dynamics, Interactions with Proteins and Functions Highly Parallel and Distributed Computing Systems
issn	1360-8185
number of pages	17 (xsd:int)
http://localhost/t...ganizacniJednotka	14330

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